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MB Sample ID: SA137224
| Local Sample ID: | CLTI Pre-surgery6B |
| Subject ID: | SU001694 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 55-82 years |
| Gender: | Male and female |
| Human Race: | unknown |
| Human Ethnicity: | unknown |
| Human Medications: | Asprin, ACE inhibitor, statins, cilostazol |
| Human Smoking Status: | Controls = 4, CLTI Pre-surgery = 7, and CLTI Amputation = 9 |
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Subject:
| Subject ID: | SU001694 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 55-82 years |
| Gender: | Male and female |
| Human Race: | unknown |
| Human Ethnicity: | unknown |
| Human Medications: | Asprin, ACE inhibitor, statins, cilostazol |
| Human Smoking Status: | Controls = 4, CLTI Pre-surgery = 7, and CLTI Amputation = 9 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| CLTI Pre-surgery6B | SA137224 | FL017532 | CLTI_Pre_surgery | Group |
Collection:
| Collection ID: | CO001687 |
| Collection Summary: | Gastrocnemius muscle specimens were obtained from ten older adult non-PAD controls (Control), ten patients with critical limb ischemia (CLI) undergoing surgical intervention (CLTI Pre-surgery), and ten CLI patients undergoing limb amputation (CLTI Amputation). Five pre-surgery patients underwent bypass interventions and five underwent endovascular procedures. Muscle specimens were collected within the confines of the operating rooms (CLI patients) or via percutaneous muscle biopsy using sterile procedures. A portion of the muscle was quickly trimmed of fat/connective tissue and snap frozen in liquid nitrogen for metabolomics analysis. |
| Sample Type: | Muscle |
| Collection Method: | Muscle specimens were collected within the confines of the operating rooms (CLI patients) or via percutaneous muscle biopsy using sterile procedures. A portion of the muscle was quickly trimmed of fat/connective tissue and snap frozen in liquid nitrogen for metabolomics analysis. |
| Storage Conditions: | -80℃ |
| Storage Vials: | cryovials |
Treatment:
| Treatment ID: | TR001707 |
| Treatment Summary: | This was a prospective cohort study that examined the metabolomic profile of skeletal muscle from CLTI patients undergoing surgerical intervention or amputation, as well as a cohort of non-PAD controls. CLTI patients exhibited severe symptomology (Rutherford Classification 3-6), with high incidence of common PAD risk factors including hypertension, hyperlipidemia, coronary artery disease, and diabetes.Ten patients with critical limb ischemia (CLI) undergoing surgical intervention (CLTI Pre-surgery), and ten CLI patients undergoing limb amputation (CLTI Amputation). Five pre-surgery patients underwent bypass interventions and five underwent endovascular procedures. Muscle specimens were collected within the confines of the operating rooms (CLI patients) or via percutaneous muscle biopsy using sterile procedures. |
| Human Fasting: | non-fasted |
| Human Endp Clinical Signs: | N/A |
Sample Preparation:
| Sampleprep ID: | SP001700 |
| Sampleprep Summary: | Semi-solid HR-MAS spectra were collected on intact human gastrocnemius muscle specimens. |
| Processing Method: | None |
| Processing Storage Conditions: | -80℃ |
| Extract Storage: | -80℃ |
| Sample Resuspension: | None |
| Sample Spiking: | None |
Analysis:
| MB Sample ID: | SA137224 |
| Analysis ID: | AN002652 |
| Laboratory Name: | Terence lab, UF |
| Analysis Type: | NMR |
| Acquisition Date: | 08/31/2020 to 09/03/2020 |
| Software Version: | Bruker Topspin |
| Operator Name: | Ram Khattri |
| Detector Type: | Bruker |
| Data Format: | fid, 1r |
| Num Factors: | 3 |
| Num Metabolites: | 25 |
| Units: | A.U. |
NMR:
| NMR ID: | NM000189 |
| Analysis ID: | AN002652 |
| Instrument Name: | Bruker Avance 800 MHz with HR-MAS console |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D-1H |
| Field Frequency Lock: | Deuterium |
| Standard Concentration: | None |
| Spectrometer Frequency: | 800 MHz |
| NMR Probe: | HR-MAS probe |
| NMR Solvent: | For HR-MAS experiments, deuterium oxide was used. |
| NMR Tube Size: | 3.2 mm inside diameter plastic insert was used for HR-MAS experiments. |
| Shimming Method: | Topshim |
| Pulse Sequence: | noesypr1d |
| Water Suppression: | presat |
| Pulse Width: | 90-degree |
| Receiver Gain: | 28.5 |
| Offset Frequency: | 4.96 Hz |
| Chemical Shift Ref Cpd: | None |
| Temperature: | 4 oC |
| Number Of Scans: | 256 |
| Dummy Scans: | 8 |
| Acquisition Time: | 2.0447 s |
| Relaxation Delay: | 2 s |
| Spectral Width: | 8012.8 Hz |
| Num Data Points Acquired: | 16384 points |
| Real Data Points: | 65536 |
| Line Broadening: | 0.5 Hz |
| Zero Filling: | 65,536 points |
| Apodization: | Exponential |
| Baseline Correction Method: | Spline |
| Chemical Shift Ref Std: | Alanine doublet at 1.46 ppm |
