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MB Sample ID: SA138079
Local Sample ID: | 1-A3-PS |
Subject ID: | SU001709 |
Subject Type: | Mammal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Genotype Strain: | Sprague-Dawley |
Age Or Age Range: | 6 weeks-age |
Weight Or Weight Range: | 200 ± 10 |
Gender: | Male |
Animal Animal Supplier: | Vital River Laboratories Co., Ltd (Beijng, China) |
Animal Housing: | 25 ± 2℃, 50±5% relative humidity |
Animal Light Cycle: | 12 h light/12 h dark photoperiod |
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Subject:
Subject ID: | SU001709 |
Subject Type: | Mammal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Genotype Strain: | Sprague-Dawley |
Age Or Age Range: | 6 weeks-age |
Weight Or Weight Range: | 200 ± 10 |
Gender: | Male |
Animal Animal Supplier: | Vital River Laboratories Co., Ltd (Beijng, China) |
Animal Housing: | 25 ± 2℃, 50±5% relative humidity |
Animal Light Cycle: | 12 h light/12 h dark photoperiod |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
1-A3-PS | SA138079 | FL017566 | Control | class |
1-A3-PS | SA138079 | FL017566 | plasma | Tissue |
Collection:
Collection ID: | CO001702 |
Collection Summary: | Blood samples of the rats were collected to obtain plasma by centrifugation (3000r/min, 15 min) using heparin as an anticoagulant and kept at -80℃ until analysis. Hearts were collected, washed with cold saline and cut in half. One part was grinded into powder in liquid nitrogen and kept at -80℃. Heart samples were homogenized in saline on ice at the ratio of tissue to solution was 1:4 (w/v) before analysis. |
Sample Type: | Heart |
Treatment:
Treatment ID: | TR001722 |
Treatment Summary: | Rats were divided into six rats in each group randomly as following: control rats group, MI model rats group, MI model rats pre-treated with diltiazem group (50 mg/kg, i.g.), and MI model rats pre-treated with GTSS group (200 mg/kg, i.g.). Pre-treated rats were consecutively administrated for 4 weeks. Except control rats group received saline, other rat groups received two injections of isoproterenol (85 mg/kg, s.c.) on the 27th and 28th days at an interval of 24 hours. |
Sample Preparation:
Sampleprep ID: | SP001715 |
Sampleprep Summary: | 100μl of plasma or tissue homogenate was transferred into a 10 ml glass tube and added 20 μl of lipid internal standards. After vortexed for 10 s, 1.5 ml of methanol and 5 ml methyl tert-butyl ether were added to the tube, and the sample was vortexed for 15 min and then added 1.5 ml water, then centrifuged at 4500r/min for 10 min (4 ℃). The upper organic phase was transferred to another glass tube, and the lower water phase was added with 2 ml secondary extractant (methyl tert-butyl ether /methanol/water,10:3:2.5, v/v/v). After vortexed for 10min and centrifuged for 15min at 4500rpm, and the upper organic phase was combined and dried under a gentle nitrogen stream. The residue was re-dissolved in 100 μl methanol/chloroform (1:1, v/v). |
Combined analysis:
Analysis ID | AN002667 | AN002668 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Agilent 1100 | Thermo Accela 1250 |
Column | Peeke C8 SR (150×3.0mm, 3μm) | Waters Xterra MS C8 (100 x 2.1mm,3.5um) |
MS Type | ESI | ESI |
MS instrument type | Triple quadrupole | LTQ-FT |
MS instrument name | Agilent 6410 QQQ | Thermo LTQ-FT |
Ion Mode | POSITIVE | UNSPECIFIED |
Units | pmol/ml(PL) & pmol/mg(HL) | pmol/ml(PS) & pmol/mg(HS) |
Chromatography:
Chromatography ID: | CH001962 |
Instrument Name: | Agilent 1100 |
Column Name: | Peeke C8 SR (150×3.0mm, 3μm) |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH001963 |
Instrument Name: | Thermo Accela 1250 |
Column Name: | Waters Xterra MS C8 (100 x 2.1mm,3.5um) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS002466 |
Analysis ID: | AN002667 |
Instrument Name: | Agilent 6410 QQQ |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | The parameters for electrospray ionization tandem MS in positive ion mode were as follows: gas temperature, 350 ℃; gas flow rate, 10 l/min; nebulizer, 30 psi; and capillary voltage, 4000V. Multiple reaction monitoring was performed using the characteristic precursor-to-product ion transitions, optimized fragmentor voltages, and collision energies. |
Ion Mode: | POSITIVE |
MS ID: | MS002467 |
Analysis ID: | AN002668 |
Instrument Name: | Thermo LTQ-FT |
Instrument Type: | LTQ-FT |
MS Type: | ESI |
MS Comments: | The LTQ-FT was run in full-scan mode at 100000 resolution from m/z50–1200 with MS parameters: sheath gas flow rate, 50 arb; aux gas flow rate, 20 arb; sweep gas flow rate, 3 arb; capillary temperature, 275 ℃ in both positive and negative mode. And spray voltage, 4.5kV; capillary voltage, 35.0V; tube lens, 120V were used for the positive mode. Spray voltage, -4.0kV; capillary voltage, -35.0V; tube lens, -120V were used for the negative mode. |
Ion Mode: | UNSPECIFIED |