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MB Sample ID: SA163212
Local Sample ID: | 321588 |
Subject ID: | SU001819 |
Subject Type: | Mammal |
Subject Species: | Canis lupus familiaris |
Taxonomy ID: | 9615 |
Age Or Age Range: | Adult |
Weight Or Weight Range: | 3-60 kg |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001819 |
Subject Type: | Mammal |
Subject Species: | Canis lupus familiaris |
Taxonomy ID: | 9615 |
Age Or Age Range: | Adult |
Weight Or Weight Range: | 3-60 kg |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
321588 | SA163212 | FL019434 | Diabetic | Group |
321588 | SA163212 | FL019434 | Fasted | Fasted vs Fed |
Collection:
Collection ID: | CO001812 |
Collection Summary: | Blood collected by peripheral venipuncture into red top tube (containing clot activator). Blood was allowed to clot, then centrifuged at 2000 g x 7.5 minutes between 25-35 minutes after sample collection. Serum was frozen immediately after separation (always within 1 hour of collection) at -80 C. |
Collection Protocol Filename: | Protein_Precipitation_Sample_Prep.pdf |
Sample Type: | Blood (serum) |
Collection Method: | Peripheral venipuncture |
Collection Location: | Jugular, saphenous, or cephalic vein |
Collection Frequency: | Once |
Volumeoramount Collected: | Variable |
Storage Conditions: | -80℃ |
Collection Vials: | Red top with clot activator |
Storage Vials: | Cryovials (polypropylene); Microcentrifuge tubes (polypropylene) for second round |
Collection Tube Temp: | Room temperature |
Treatment:
Treatment ID: | TR001832 |
Treatment Summary: | None for research study. Variable medications may have been received by diabetic dogs. Healthy dogs are only allowed to receive flea/tick/heartworm preventatives. |
Sample Preparation:
Sampleprep ID: | SP001825 |
Sampleprep Summary: | TRP Quant_Protein Precipitation Sample Prep.docx |
Sampleprep Protocol Filename: | Protein_Precipitation_Sample_Prep.pdf |
Combined analysis:
Analysis ID | AN002835 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 |
Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Plus Orbitrap |
Ion Mode | POSITIVE |
Units | ng/mL |
Chromatography:
Chromatography ID: | CH002098 |
Chromatography Summary: | Protein Precipitation Sample Prep.pdf |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS002628 |
Analysis ID: | AN002835 |
Instrument Name: | Thermo Q Exactive Plus Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Protein Precipitation Sample Prep.pdf |
Ion Mode: | POSITIVE |
Analysis Protocol File: | Protein_Precipitation_Sample_Prep.pdf |