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MB Sample ID: SA163259
| Local Sample ID: | 287971 |
| Subject ID: | SU001819 |
| Subject Type: | Mammal |
| Subject Species: | Canis lupus familiaris |
| Taxonomy ID: | 9615 |
| Age Or Age Range: | Adult |
| Weight Or Weight Range: | 3-60 kg |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001819 |
| Subject Type: | Mammal |
| Subject Species: | Canis lupus familiaris |
| Taxonomy ID: | 9615 |
| Age Or Age Range: | Adult |
| Weight Or Weight Range: | 3-60 kg |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 287971 | SA163259 | FL019437 | Healthy | Group |
| 287971 | SA163259 | FL019437 | Fed | Fasted vs Fed |
Collection:
| Collection ID: | CO001812 |
| Collection Summary: | Blood collected by peripheral venipuncture into red top tube (containing clot activator). Blood was allowed to clot, then centrifuged at 2000 g x 7.5 minutes between 25-35 minutes after sample collection. Serum was frozen immediately after separation (always within 1 hour of collection) at -80 C. |
| Collection Protocol Filename: | Protein_Precipitation_Sample_Prep.pdf |
| Sample Type: | Blood (serum) |
| Collection Method: | Peripheral venipuncture |
| Collection Location: | Jugular, saphenous, or cephalic vein |
| Collection Frequency: | Once |
| Volumeoramount Collected: | Variable |
| Storage Conditions: | -80℃ |
| Collection Vials: | Red top with clot activator |
| Storage Vials: | Cryovials (polypropylene); Microcentrifuge tubes (polypropylene) for second round |
| Collection Tube Temp: | Room temperature |
Treatment:
| Treatment ID: | TR001832 |
| Treatment Summary: | None for research study. Variable medications may have been received by diabetic dogs. Healthy dogs are only allowed to receive flea/tick/heartworm preventatives. |
Sample Preparation:
| Sampleprep ID: | SP001825 |
| Sampleprep Summary: | TRP Quant_Protein Precipitation Sample Prep.docx |
| Sampleprep Protocol Filename: | Protein_Precipitation_Sample_Prep.pdf |
Combined analysis:
| Analysis ID | AN002835 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 |
| Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Plus Orbitrap |
| Ion Mode | POSITIVE |
| Units | ng/mL |
Chromatography:
| Chromatography ID: | CH002098 |
| Chromatography Summary: | Protein Precipitation Sample Prep.pdf |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS002628 |
| Analysis ID: | AN002835 |
| Instrument Name: | Thermo Q Exactive Plus Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Protein Precipitation Sample Prep.pdf |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | Protein_Precipitation_Sample_Prep.pdf |
