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MB Sample ID: SA163980
| Local Sample ID: | 328335 |
| Subject ID: | SU001831 |
| Subject Type: | Mammal |
| Subject Species: | Canis lupus familiaris |
| Taxonomy ID: | 9615 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001831 |
| Subject Type: | Mammal |
| Subject Species: | Canis lupus familiaris |
| Taxonomy ID: | 9615 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 328335 | SA163980 | FL019527 | Healthy | Diabetic or Healthy |
| 328335 | SA163980 | FL019527 | MN | Sex |
| 328335 | SA163980 | FL019527 | Fed | Fasted or Fed |
| 328335 | SA163980 | FL019527 | Miniature Australian Shepherd | Breed |
Collection:
| Collection ID: | CO001824 |
| Collection Summary: | Blood collected by peripheral venipuncture into red top tube (containing clot activator). Blood was allowed to clot, then centrifuged at 2000 g x 7.5 minutes between 20-30 minutes after sample collection. Serum was frozen immediately after separation (always within 1 hour of collection) at -80 C. |
| Sample Type: | Blood (serum) |
Treatment:
| Treatment ID: | TR001844 |
| Treatment Summary: | None for research study. Variable medications may have been received by diabetic dogs. Healthy dogs are only allowed to receive flea/tick/heartworm preventatives. |
Sample Preparation:
| Sampleprep ID: | SP001837 |
| Sampleprep Summary: | see protocol attached |
| Sampleprep Protocol Filename: | Protein_Precipitation_Sample_Prep.pdf |
Combined analysis:
| Analysis ID | AN002857 | AN002858 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 RS | Thermo Dionex Ultimate 3000 RS |
| Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Exactive Plus Orbitrap | Thermo Exactive Plus Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak Height | Peak Height |
Chromatography:
| Chromatography ID: | CH002116 |
| Chromatography Summary: | see attached |
| Instrument Name: | Thermo Dionex Ultimate 3000 RS |
| Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS002650 |
| Analysis ID: | AN002857 |
| Instrument Name: | Thermo Exactive Plus Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | see attached |
| Ion Mode: | POSITIVE |
| MS ID: | MS002651 |
| Analysis ID: | AN002858 |
| Instrument Name: | Thermo Exactive Plus Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | see attached |
| Ion Mode: | NEGATIVE |
