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MB Sample ID: SA163991
Local Sample ID: | 274821 |
Subject ID: | SU001831 |
Subject Type: | Mammal |
Subject Species: | Canis lupus familiaris |
Taxonomy ID: | 9615 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001831 |
Subject Type: | Mammal |
Subject Species: | Canis lupus familiaris |
Taxonomy ID: | 9615 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
274821 | SA163991 | FL019537 | Healthy | Diabetic or Healthy |
274821 | SA163991 | FL019537 | SF | Sex |
274821 | SA163991 | FL019537 | Fed | Fasted or Fed |
274821 | SA163991 | FL019537 | Miniature Schnauzer | Breed |
Collection:
Collection ID: | CO001824 |
Collection Summary: | Blood collected by peripheral venipuncture into red top tube (containing clot activator). Blood was allowed to clot, then centrifuged at 2000 g x 7.5 minutes between 20-30 minutes after sample collection. Serum was frozen immediately after separation (always within 1 hour of collection) at -80 C. |
Sample Type: | Blood (serum) |
Treatment:
Treatment ID: | TR001844 |
Treatment Summary: | None for research study. Variable medications may have been received by diabetic dogs. Healthy dogs are only allowed to receive flea/tick/heartworm preventatives. |
Sample Preparation:
Sampleprep ID: | SP001837 |
Sampleprep Summary: | see protocol attached |
Sampleprep Protocol Filename: | Protein_Precipitation_Sample_Prep.pdf |
Combined analysis:
Analysis ID | AN002857 | AN002858 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 RS | Thermo Dionex Ultimate 3000 RS |
Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Exactive Plus Orbitrap | Thermo Exactive Plus Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | Peak Height | Peak Height |
Chromatography:
Chromatography ID: | CH002116 |
Chromatography Summary: | see attached |
Instrument Name: | Thermo Dionex Ultimate 3000 RS |
Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS002650 |
Analysis ID: | AN002857 |
Instrument Name: | Thermo Exactive Plus Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | see attached |
Ion Mode: | POSITIVE |
MS ID: | MS002651 |
Analysis ID: | AN002858 |
Instrument Name: | Thermo Exactive Plus Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | see attached |
Ion Mode: | NEGATIVE |