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MB Sample ID: SA171648
| Local Sample ID: | 840422-24_016 |
| Subject ID: | SU001922 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 23-73 |
| Gender: | Male and female |
| Human Race: | Hispanic and Caucasian |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001922 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 23-73 |
| Gender: | Male and female |
| Human Race: | Hispanic and Caucasian |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 840422-24_016 | SA171648 | FL020628 | NAFLD-CAU | factor |
Collection:
| Collection ID: | CO001915 |
| Collection Summary: | Blood was collected as a part of a routine/pre-operation check up, not more than 2 weeks prior to operation day (bariatric surgery) for the NAFLD group and among normal BMI healthy volunteers for the control group. All volunteers were fasted 10-12 hours before collection. Liver obtained during surgery (no preservatives, flash-frozen) |
| Sample Type: | Blood |
Treatment:
| Treatment ID: | TR001934 |
| Treatment Summary: | Subjects were divided into two groups either: Healthy control or Nonalcoholic fatty liver disease (NAFLD) |
Sample Preparation:
| Sampleprep ID: | SP001928 |
| Sampleprep Summary: | 1 Thaw plasma samples on ice 2 Vortex plasma sample and pipette 50µl in polypropylene Eppendorf tube/Plate 3 Add Surrogate Standards of the 3 lipid classes (as needed) and other reagents as in the following table: Plasma Sample 10x Oxylipid Surrogate STDs CUDA + PHAU Anti-oxidant ACN/MeOH SUM 4 Vortex for 30sec 5 Centrifuge at 6°C for 5 min at 15,000 g (or 1,800 g for plates) 6 Take the supernatant (~240µl) into new Eppendorf tube/filter plate 7 Spin Filter (0.1 um) or plate filter (0.2um) for 5 min @ 12,000 g (1,800g for plate) 8 place aliquotes of samples into vials, or plate, and cap vials or seal plates 9 Store in -20°C until analysis |
Combined analysis:
| Analysis ID | AN002988 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Thermo Vanquish |
| Column | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
| MS Type | EI |
| MS instrument type | Other |
| MS instrument name | ABI Sciex 4000 QTrap |
| Ion Mode | UNSPECIFIED |
| Units | nM |
Chromatography:
| Chromatography ID: | CH002217 |
| Chromatography Summary: | Targeted oxylipin analysis |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS002778 |
| Analysis ID: | AN002988 |
| Instrument Name: | ABI Sciex 4000 QTrap |
| Instrument Type: | Other |
| MS Type: | EI |
| MS Comments: | analyzed by a Waters UPLC with a 2.1×150 mm, 1.7 µm Acquity BEH column, ESI (+) and (-) switching and detection by multi-reaction monitoring on an Sciex 6500+ QTRAP mass spectrometer. We validated this method for linearity, limit of detection, limit of quantitation, inter- and intra-day reproducibility, and recovery for all targets, yielding better than 15% relative standard deviation and (spiked) recoveries of greater than 80%. |
| Ion Mode: | UNSPECIFIED |
