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MB Sample ID: SA171778
| Local Sample ID: | GM60 |
| Subject ID: | SU001924 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001924 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| GM60 | SA171778 | FL020640 | Facility1 | Facility |
| GM60 | SA171778 | FL020640 | GM15 | Group |
| GM60 | SA171778 | FL020640 | F | Sex |
| GM60 | SA171778 | FL020640 | Polar | Analysis |
Collection:
| Collection ID: | CO001917 |
| Collection Summary: | Mice were killed by cervical dislocation and biocollections were performed aseptically. Plasma was collected by centrifugation from lithium heparin blood. |
| Sample Type: | Blood (plasma) |
Treatment:
| Treatment ID: | TR001936 |
| Treatment Summary: | SOPF (C57BL/6J ) mice were obtained from Charles River Lab GF (C57BL/6J ) mice were produced in-house by aseptic hysterectomy of a SOPF (C57BL/6J) female GM15 founders were 8-week-old GF (C57BL/6J) mice colonized by oral inoculations of the GM15 bacterial community: Bacteroides acidifaciens MD185 Bacteroides caecimuris MD237 Parabacteroides goldsteinii MD072 Clostridium cocleatum I50 Enterocloster clostridioformis YL32 Clostridium sp. MD294 Clostridium sp. MD300 Subtilibacillum caecimuris MD335 Longibacillum caecimuris MD329 Irregularicoccus caecimuris MD308 Lactobacillus johnsonii MD006 Ligilactobacillus murinus MD040 Limosilactobacillus reuteri MD207 Anaerotruncus colihominis JM4-15 Escherichia coli Mt1B1 |
Sample Preparation:
| Sampleprep ID: | SP001930 |
| Sampleprep Summary: | Plasma was deproteinized by acid ultrafiltration using 10kDA filter tubes. The lipids were extracted from the same sample after ultrafiltration. |
Analysis:
| MB Sample ID: | SA171778 |
| Analysis ID: | AN002991 |
| Laboratory Name: | Bioaster |
| Analysis Type: | NMR |
| Software Version: | Topspin 3.6.2 |
| Operator Name: | Andrei Bunescu |
| Data Format: | FID |
| Num Factors: | 24 |
| Num Metabolites: | 62 |
| Units: | mM |
NMR:
| NMR ID: | NM000207 |
| Analysis ID: | AN002991 |
| Instrument Name: | Ascend HD III 600MHz Bruker |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D-1H |
| Spectrometer Frequency: | 600MHz |
| NMR Probe: | 5mm QCI 1H, 13C, 15N, 31P |
| NMR Solvent: | H2O:D2O |
| NMR Tube Size: | 3mm |
| Shimming Method: | topshim |
| Pulse Sequence: | noesygppr1d and for lipids zg30 |
