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MB Sample ID: SA174220
Local Sample ID: | b5 |
Subject ID: | SU001932 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6 |
Age Or Age Range: | 8 weeks |
Weight Or Weight Range: | 20-25 g |
Gender: | Male |
Animal Animal Supplier: | Model Animal Research Center of Nanjing University |
Animal Housing: | standard specific pathogen free (SPF) conditions |
Animal Light Cycle: | 12:12-h light/dark cycle |
Animal Feed: | food freely |
Animal Water: | water freely |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001932 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6 |
Age Or Age Range: | 8 weeks |
Weight Or Weight Range: | 20-25 g |
Gender: | Male |
Animal Animal Supplier: | Model Animal Research Center of Nanjing University |
Animal Housing: | standard specific pathogen free (SPF) conditions |
Animal Light Cycle: | 12:12-h light/dark cycle |
Animal Feed: | food freely |
Animal Water: | water freely |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
b5 | SA174220 | FL021370 | UIR | surgery |
b5 | SA174220 | FL021370 | - | FG-4592(10 mg/kg) |
Collection:
Collection ID: | CO001925 |
Collection Summary: | Non-target metabolomics analysis was carried out using the kidney tissues of mice sacrificed at day 21 after UIR. |
Sample Type: | Kidney |
Treatment:
Treatment ID: | TR001944 |
Treatment Summary: | C57BL/6 mice were anesthetized using isoflurane. UIR was induced by clamping the right renal pedicle for 45 minutes and then releasing it to allow reperfusion, leaving the left kidney intact. Sham treated mice served as controls. Each mouse was located supine on a thermostatic pad (37 °C) to maintain its body temperature throughout the whole process. After 3 days of recovery, the mice received a daily intraperitoneal (i.p.) injection of FG4592 (10 mg/kg) or vehicle for 18 consecutive days. After treatment, the mice were sacrificed. Non-target metabolomics analysis was carried out using the kidney tissues of mice sacrificed at day 21 after UIR. |
Treatment Compound: | FG4592 |
Treatment Dose: | 10 mg/kg |
Sample Preparation:
Sampleprep ID: | SP001938 |
Sampleprep Summary: | 20 mg of each tissue sample were homogenized with 0.6 mL of 2 chloro phenylalanine (4 ppm) containing methanol (−20 °C), centrifuged, and filtered through a 0.22-μm membrane |
Combined analysis:
Analysis ID | AN003006 | AN003007 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Ultimate 3000 system | Thermo Ultimate 3000 system |
Column | Waters ACQUITY UPLC HSS T3 | Waters ACQUITY UPLC HSS T3 |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | AUC | AUC |
Chromatography:
Chromatography ID: | CH002229 |
Chromatography Summary: | A 2 μL aliquot of the samples was injected into a Thermo Ultimate 3000 system equipped with an ACQUITY UPLC® HSS T3 column (150 × 2.1 mm, 1.8 μm, Waters, Milford, MA, USA) maintained at 40 °C. The mobile phase for positive mode analysis consists of 0.1% formic acid in water (phase C) and 0.1% formic acid in acetonitrile (phase D), while the mobile phase for negative mode analysis consists of 5 mM ammonium formate in water (phase A) and acetonitrile (phase B). Phase C and D, as well as phase A and B, were mixed and delivered at a flow rate of 0.25 mL/min with a gradient program as follows: 0~1 min; 2% B/D; 1~9 min, 2%~50% B/D; 9~12 min, 50%~98% B/D; 12~13.5 min, 98% B/D; 13.5~14 min, 98%~2% B/D; 14~20 min, 2% D-positive model (14~17 min, 2% B-negative model). |
Instrument Name: | Thermo Ultimate 3000 system |
Column Name: | Waters ACQUITY UPLC HSS T3 |
Column Temperature: | 40 |
Flow Gradient: | phase A and B were mixed and delivered at a flow rate of 0.25 mL/min with a gradient program as follows: 0-1 min; 2% A/B; 1-9 min, 2%-50% A/B; 9-12 min, 50%-98% A/B; 12-13.5 min, 98% A/B; 13.5-14 min, 98%-2% A/B; 14-20 min, 2% B-positive model (14-17 min, 2% B-negative model) |
Flow Rate: | 0.25ml/min |
Solvent A: | 100% water; 5mM ammonium formate |
Solvent B: | 100% acetonitrile |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS002795 |
Analysis ID: | AN003006 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Proteowizard software (v3.0.8789) |
Ion Mode: | POSITIVE |
MS ID: | MS002796 |
Analysis ID: | AN003007 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Proteowizard software (v3.0.8789) |
Ion Mode: | NEGATIVE |