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MB Sample ID: SA174851
Local Sample ID: | Mouse faeces-15V |
Subject ID: | SU001952 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001952 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
Mouse faeces-15V | SA174851 | FL021483 | Mouse feces | Source |
Mouse faeces-15V | SA174851 | FL021483 | 15V | MS2 CE |
Collection:
Collection ID: | CO001945 |
Collection Summary: | Faecal samples and liver tissue were collected and immediately stored at -80 °C for further analysis. |
Sample Type: | Mouse feces and liver |
Storage Conditions: | -80℃ |
Treatment:
Treatment ID: | TR001964 |
Treatment Summary: | No treatment |
Sample Preparation:
Sampleprep ID: | SP001958 |
Sampleprep Summary: | Mouse liver tissue: Six- to eight-week-old male C57BL/6 mice were fed a regular standard breeding diet and housed under standard pathogen-free (SPF) conditions. Faecal samples were collected and immediately stored at -80 °C for further analysis. After the mice were sacrificed, liver tissue was sampled, immediately frozen in liquid nitrogen, and then stored at -80 °C. The study was approved by the Animal Ethics Committee of Saiye Biology (ACU20-A027). Mouse liver tissue (20 mg) and 600 μL CH3OH/H2O (8:2, vol/vol) solvent were combined in a centrifuge tube and then homogenized (28 Hz, 60 s and 30 s) in a frozen mixed ball grinding machine (MM400, Retsch Technology, Han, Germany). The mixture was centrifuged for 10 min at 15,000 g and 4 °C, 500 μL of supernatant was transferred to a new centrifuge tube for lyophilization, and the residue was reconstituted with 100 μL of Mix-IS-MeOH.Mouse faeces: A 60 mg sample of mouse faeces and 600 μL CH3OH/H2O (8:2, vol/vol) solvent were combined in a centrifuge tube and then homogenized (28 Hz, 60 s and 30 s) in a frozen mixed ball grinding machine (MM400, Retsch Technology, Han, Germany). The mixture was centrifuged for 10 min at 15,000 g and 4 °C; then, 500 μL of supernatant was transferred to a new centrifuge tube for lyophilization, and the residue was reconstituted with 100 μL of Mix-IS-MeOH. |
Combined analysis:
Analysis ID | AN003037 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Waters Acquity |
Column | Waters Acquity BEH C8 (100 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | ABI Sciex 5600+ TripleTOF |
Ion Mode | POSITIVE |
Units | blank-substracted abundances |
Chromatography:
Chromatography ID: | CH002250 |
Instrument Name: | Waters Acquity |
Column Name: | Waters Acquity BEH C8 (100 x 2.1mm,1.7um) |
Column Temperature: | 50 |
Flow Rate: | 0.35 mL/min |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS002824 |
Analysis ID: | AN003037 |
Instrument Name: | ABI Sciex 5600+ TripleTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | The spray voltage was 5.5 kV and the source temperature was 550 °C, Curtain gas, gas 1 and gas 2 were set at 35, 55, and 55 psi, respectively. The data was processed by XCMS, MS-DIAL, MZmine 2 and MetEx. |
Ion Mode: | POSITIVE |