Return to study ST001884 main page
MB Sample ID: SA175149
| Local Sample ID: | 44_3k |
| Subject ID: | SU001962 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | Ndufs4, https://www.jax.org/strai n/02705 8 |
| Age Or Age Range: | 45-50 days |
| Gender: | Male |
| Animal Animal Supplier: | Jackson Laboratory (ME, USA) |
| Animal Light Cycle: | 12:12 h |
| Animal Feed: | Rodent Breeder, Cat. #RM1845, LabChef, Nutritionhub |
| Animal Water: | ad libitum |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001962 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | Ndufs4, https://www.jax.org/strai n/02705 8 |
| Age Or Age Range: | 45-50 days |
| Gender: | Male |
| Animal Animal Supplier: | Jackson Laboratory (ME, USA) |
| Animal Light Cycle: | 12:12 h |
| Animal Feed: | Rodent Breeder, Cat. #RM1845, LabChef, Nutritionhub |
| Animal Water: | ad libitum |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 44_3k | SA175149 | FL021511 | KO | Genotype |
Collection:
| Collection ID: | CO001955 |
| Collection Summary: | Urine was collected from individual mice in metabolic cages after overnight (12 hours) fasting with ad libitum access to water. Since Ndufs4 KO mice yielded limited urine volumes, urine droplets that failed to reach the collection tube were also collected. Hence, the separating cone, collection funnel and urine collection tubes were all treated with sodium azide (NaN3; 0.1% w/v; WT: 100 μL and KO: 50 μL) to prevent bacterial growth. Samples were snap-frozen in liquid nitrogen upon collection and stored at −80 °C until used. |
| Sample Type: | Urine |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR001974 |
| Treatment Summary: | The animals did not receive any treatment |
Sample Preparation:
| Sampleprep ID: | SP001968 |
| Sampleprep Summary: | Pre-determined volumes of urine, containing 0.0625 μmole creatinine, were supplemented with internal standards (included stable isotopes) and deproteinized using methanol. Samples were then dried under nitrogen and butylated prior to analysis. |
Combined analysis:
| Analysis ID | AN003048 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1200 |
| Column | Agilent C18 Zorbax SB-AQ (150 x 2.1mm, 3.5um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Agilent 6410 QQQ |
| Ion Mode | POSITIVE |
| Units | mg/g creatinine |
Chromatography:
| Chromatography ID: | CH002258 |
| Instrument Name: | Agilent 1200 |
| Column Name: | Agilent C18 Zorbax SB-AQ (150 x 2.1mm, 3.5um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS002835 |
| Analysis ID: | AN003048 |
| Instrument Name: | Agilent 6410 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | The Agilent© MassHunter Workstation (v B02.01) and MassHunter Optimiser (v B02.01) software were used for data acquisition in the multiple reaction monitoring (MRM) configuration setting with parameters set for target amino acids, -derivatives, acylcarnitines, and isotopes. Data extraction was done using the Agilent© MassHunter Workstation software (v B06.00; Qualitative Analysis and Quantitative Analysis) |
| Ion Mode: | POSITIVE |
