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MB Sample ID: SA177707
| Local Sample ID: | KO_ZT22_1 |
| Subject ID: | SU001993 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001993 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| KO_ZT22_1 | SA177707 | FL021968 | KO_ZT22 | Group |
| KO_ZT22_1 | SA177707 | FL021968 | Rev-erb cardiomyocyte specific knock out | Genotype |
| KO_ZT22_1 | SA177707 | FL021968 | KO mouse heart ventrcle harvest at ZT22 | Experimental variables |
Collection:
| Collection ID: | CO001986 |
| Collection Summary: | Snap-frozen heart ventricles were collected from both WT and Rev-erb cardiomyocyte-specific KO mice at ZT10 or ZT22 |
| Sample Type: | Heart |
Treatment:
| Treatment ID: | TR002005 |
| Treatment Summary: | no special treatment |
Sample Preparation:
| Sampleprep ID: | SP001999 |
| Sampleprep Summary: | Heart ventricle tissues were harvested from male mice at 3 months old (n = 3 at each condition) and were snap-frozen in liquid nitrogen. Lipids were extracted as previously described 55–57. Lipids were separated on a Shimadzu CTO-20A Nexera X2 UHPLC systems, 1.8 μm particle 50 × 2.1 mm Acquity HSS UPLC T3 column (Waters, Milford, MA). Lipidomics acquisition performed in both pos and neg mode ionization. Pos mode lipids were normalized by Internal Standard PC 17:0; [M+H]+, Neg mode lipids were normalized by Internal Standard PC 34:0. |
Combined analysis:
| Analysis ID | AN003113 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Shimadzu Nexera X2 |
| Column | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | EI |
| MS instrument type | Other |
| MS instrument name | Shimadzu QP2010 Plus |
| Ion Mode | UNSPECIFIED |
| Units | pmoles/l |
Chromatography:
| Chromatography ID: | CH002298 |
| Instrument Name: | Shimadzu Nexera X2 |
| Column Name: | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS002894 |
| Analysis ID: | AN003113 |
| Instrument Name: | Shimadzu QP2010 Plus |
| Instrument Type: | Other |
| MS Type: | EI |
| MS Comments: | Statistical analyses were performed with either ANOVA or t-test in R Studio (R Studio Inc., Boston, MA). Differential metabolites were identified by adjusting the p-values for multiple testing at an FDR (Benjamini Hochberg method) threshold of <0.25. |
| Ion Mode: | UNSPECIFIED |
