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MB Sample ID: SA183515

Local Sample ID:B07
Subject ID:SU002024
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Age Or Age Range:19-79
Weight Or Weight Range:49-93
Height Or Height Range:1.5-1.9
Gender:Male and female
Human Ethnicity:Brazilian
Human Medications:BD patients used a wide range of different medications, mostly mood stabilizers and antipsychotics
Human Inclusion Criteria:Controls: Any adult over 18 years respecting the excusion criteria / BD: Any adult over 18 years old with BD diagnosed by a psychiatrist, respecting exclusion criteria psychiat
Human Exclusion Criteria:Controls : negative history of any psychiatric disorder or use of any kind of psychiatric medication / BD : concomitant diseases such as AIDS, hepatitis, endocrinal or metabolic diseases, substance use (except nicotine dependence), and pregnancy or postpartum period

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Subject:

Subject ID:SU002024
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Age Or Age Range:19-79
Weight Or Weight Range:49-93
Height Or Height Range:1.5-1.9
Gender:Male and female
Human Ethnicity:Brazilian
Human Medications:BD patients used a wide range of different medications, mostly mood stabilizers and antipsychotics
Human Inclusion Criteria:Controls: Any adult over 18 years respecting the excusion criteria / BD: Any adult over 18 years old with BD diagnosed by a psychiatrist, respecting exclusion criteria psychiat
Human Exclusion Criteria:Controls : negative history of any psychiatric disorder or use of any kind of psychiatric medication / BD : concomitant diseases such as AIDS, hepatitis, endocrinal or metabolic diseases, substance use (except nicotine dependence), and pregnancy or postpartum period

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
B07SA183515FL022351BipolarCondition

Collection:

Collection ID:CO002017
Collection Summary:The blood was collected in a Vacutainer tube, left at ambient temperature for 30 minutes, and then stored on ice for the blood to coagulate. Afterward, the samples were centrifuged at 3500 g for 15 minutes at 4 °C. The blood serum was collected and transferred to microtubes, stored at -80 °C for further analysis.
Sample Type:Blood (serum)
Storage Conditions:-80℃

Treatment:

Treatment ID:TR002036
Treatment Summary:Samples were aliquoted into 100uL microtubes, and treated with sodium azide 0.01% (m/v), then stored in biofreezer at -80 °C until further analysis.

Sample Preparation:

Sampleprep ID:SP002030
Sampleprep Summary:The metabolites from serum samples were extracted by a simple protein precipitation method, using 50 µL of serum and 150 µL of MeOH and 150 µL of pentadecanoic acid as the internal standard. The mixture was vortexed, centrifuged at 15000 RPM, subsequently separating and discarding the protein phase. The aqueous phase was then dried in a SpeedVac at room temp and the dried samples were then submitted to a two-step derivatization, where 10 µL of methoxamine hydrochloride pyridine solution (40 mg mL-1) was added to the sample, kept at 30 ºC for 90 minutes. After the first derivatization step, 90 µL of MSTFA with 1 % (v/v) of TMCS (trimethylchlorosilane) was added, with the sample being kept at 37 ºC for 30 minutes.

Combined analysis:

Analysis ID AN003168
Analysis type MS
Chromatography type GC
Chromatography system HP/Agilent 5890 Series II
Column HP5-MS (30m x 0.25mm x 0.25um) + Duragard precolumn (10m)
MS Type EI
MS instrument type Single quadrupole
MS instrument name HP 5970 Series Quadrupole Mass Selective Detector
Ion Mode UNSPECIFIED
Units Intensities after normalization by Internal standard and log2 transformed

Chromatography:

Chromatography ID:CH002341
Chromatography Summary:The following GC/MS conditions were used. An HP 5890 Series II oven was ramped by 10°C/min from 60°C (1 min initial time) to 325°C (10 min final time), resulting in a 37.5 min run time with cooling down to 60°C. 10 µL was injected into the Agilent split/splitless injector at 250°C in splitless mode. A 30 m long HP5-MS column with 10 m Duragard precolumn was used with 0.25 mm diameter and 0.25 µm film thickness. A constant flow rate of 1 mL/min helium was used as carrier gas. The quadrupole mass spectrometer was switched on after a 5.90 min solvent delay time, scanning from 50-600 u. The source temperature was set to 230°C and the quadrupole temperature was 150°C.
Instrument Name:HP/Agilent 5890 Series II
Column Name:HP5-MS (30m x 0.25mm x 0.25um) + Duragard precolumn (10m)
Chromatography Type:GC

MS:

MS ID:MS002946
Analysis ID:AN003168
Instrument Name:HP 5970 Series Quadrupole Mass Selective Detector
Instrument Type:Single quadrupole
MS Type:EI
MS Comments:The GC-MS conditions were the same as required to use the FiehnLib library (Kind et al., 2009). The data obtained were exported as ‘.AIA’ format in GC Chemstation then transformed in ‘.d’ using Agilent GC-MS translator and finally converted in ‘.abf’ format using MS-DIAL ABF file converter. The ‘.abf’ files were then analyzed in MS-DIAL (version 4.16) for peak picking, deconvolution and peak identification. The peak table containing the identifications from FiehnLib and the respective intensities in total ion chromatogram (TIC) from each metabolite were exported and analyzed using R statistical programming language (version 3.60) to evaluate the relative standard deviations (RSD) from each metabolite on the QC samples. Metabolites with RSD > 30 % were discarded due to excessive deviation through the batch. We opted to perform a second curation of the identifications using Golm Metabolite Database (GMD) since was observed a deviation of ± 0.2 minutes on the identifications comparing to the value shown in the library, caused possibly by the equipment. Metabolites that presented inconsistencies between both platforms were considered unknown, where some of these were just identified by their functional groups using one of GMD functionalities.
Ion Mode:UNSPECIFIED
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