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MB Sample ID: SA184087
Local Sample ID: | V6 |
Subject ID: | SU002031 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | 20-53 |
Gender: | Male and female |
Cell Strain Details: | Primary isolated human pancreatic islets |
Cell Primary Immortalized: | primary |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002031 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | 20-53 |
Gender: | Male and female |
Cell Strain Details: | Primary isolated human pancreatic islets |
Cell Primary Immortalized: | primary |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
V6 | SA184087 | FL022658 | Vehicle | Group |
V6 | SA184087 | FL022658 | PBS | Treatment |
Collection:
Collection ID: | CO002024 |
Collection Summary: | Untargeted MS with annotated ions and their signal intensities |
Collection Protocol ID: | Methods Fu et al. FIA_MS |
Collection Protocol Filename: | Methods Fu et al. FIA_MS |
Sample Type: | Cultured cells |
Storage Conditions: | -80℃ |
Treatment:
Treatment ID: | TR002043 |
Treatment Summary: | Human islets were treated with vehicle DMSO 0.5% or 5uM peptide BAD SAHBa SD for 5 hours, then treated with PBS as control and inflammatory cytokines as experimental groups. |
Treatment Protocol Filename: | Methods Fu et al. FIA_MS |
Treatment Compound: | BAD SAHB SD peptide, inflammatory cytokines |
Treatment Dose: | 5 uM peptide, 10 ng/ml TNF-α, 10 ng/ml IL-1β, and 100 ng/ml IFN |
Treatment Doseduration: | 24 hours |
Treatment Vehicle: | DMSO and PBS |
Cell Media: | PIMS, Prodo Labs |
Sample Preparation:
Sampleprep ID: | SP002037 |
Sampleprep Summary: | 150 human islets per replicate were extracted in hot 70% ethanol at 75˚C for 3 min, placed on ice, and clarified by centrifugation. Extracts from all donors were pooled and analyzed in 8 replicates by flow-injections. |
Sampleprep Protocol Filename: | Methods Fu et al. FIA_MS |
Extraction Method: | Islet were extracted with hot 70% ethanol at 75˚C for 3 min |
Combined analysis:
Analysis ID | AN003178 |
---|---|
Analysis type | MS |
Chromatography type | None (Direct infusion) |
Chromatography system | Agilent 6550 Quadrupole TOF MS |
Column | none |
MS Type | ESI |
MS instrument type | Single quadrupole |
MS instrument name | Agilent 6550 QTOF |
Ion Mode | NEGATIVE |
Units | Ion signal intensity |
Chromatography:
Chromatography ID: | CH002350 |
Instrument Name: | Agilent 6550 Quadrupole TOF MS |
Column Name: | none |
Sample Injection: | 1.5 ul |
Chromatography Type: | None (Direct infusion) |
MS:
MS ID: | MS002956 |
Analysis ID: | AN003178 |
Instrument Name: | Agilent 6550 QTOF |
Instrument Type: | Single quadrupole |
MS Type: | ESI |
MS Comments: | Data acquisitions performed as a service of General Metabolics, processing and ion identification/ annotations performed with proprietary of General Metabolics. |
Ion Mode: | NEGATIVE |