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MB Sample ID: SA184361
| Local Sample ID: | G3-03 |
| Subject ID: | SU002036 |
| Subject Type: | Bacteria |
| Subject Species: | Rubrivivax benzoatilyticus |
| Taxonomy ID: | 987059 |
| Genotype Strain: | JA2 |
| Age Or Age Range: | 3, 9 and 18 days |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002036 |
| Subject Type: | Bacteria |
| Subject Species: | Rubrivivax benzoatilyticus |
| Taxonomy ID: | 987059 |
| Genotype Strain: | JA2 |
| Age Or Age Range: | 3, 9 and 18 days |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| G3-03 | SA184361 | FL022677 | 1-Exponential phase | Label |
| G3-03 | SA184361 | FL022677 | 3 | Days |
Collection:
| Collection ID: | CO002029 |
| Collection Summary: | Cells were harvested by centrifugation and the spent media was used for the metabolome analysis. |
| Sample Type: | Bacterial cells |
Treatment:
| Treatment ID: | TR002048 |
| Treatment Summary: | Bacterial cells were grown on D-glucose (22 mM) for 18 days under photoheterotrophic conditions. Cells were harvested at 3rd, 9th and 18th day of growth by centrifugation. The spent media of the bacterial cell culture was evaporated to dryness and used for the footprint analysis using GC-MS. |
| Treatment Compound: | D-glucose (dextrose) |
| Treatment Dose: | 22 mM |
| Treatment Doseduration: | 18 days |
Sample Preparation:
| Sampleprep ID: | SP002042 |
| Sampleprep Summary: | metabolites were extracted from dried culture supernatant in 1 ml methanol. Methanol extract (100 µl) was vacuum dried. The dried exometabolome samples were derivatized, to protect their functional groups, with 40 µl BSTFA (N,O-bis(trimethylsilyl)trifluoroacetamide) and TMCS (trimethylchlorosilane); incubated at 70 ○C for 4 h in dry bath and immediately analysed by GC-MS. |
| Processing Storage Conditions: | -20℃ |
Combined analysis:
| Analysis ID | AN003188 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 7890A |
| Column | Agilent HP5-MS (30m x 0.25mm, 0.25 um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 7890A |
| Ion Mode | UNSPECIFIED |
| Units | peak area |
Chromatography:
| Chromatography ID: | CH002356 |
| Chromatography Summary: | Derivatized sample (1 µl) was analysed using HP-5 column (30 m, thickness 0.25 µm) in spitless mode with helium as carrier gas at a constant flow of 1.5 ml.min-1. Oven temperature was initially held at 70○C for 2 min: ramped to 250 ○C by 10○C min-1; held for 1 min finally ramped to 280 ○C by 5○C min-1 and isocratic hold for 15 min (280 ○C). |
| Instrument Name: | Agilent 7890A |
| Column Name: | Agilent HP5-MS (30m x 0.25mm, 0.25 um) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS002966 |
| Analysis ID: | AN003188 |
| Instrument Name: | Agilent 7890A |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Metabolic features were identified based on mass spectra comparison in the database (NIST similarity >700, Golm database). |
| Ion Mode: | UNSPECIFIED |
