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MB Sample ID: SA184626
Local Sample ID: | 12B |
Subject ID: | SU002041 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Gender: | Male |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002041 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Gender: | Male |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
12B | SA184626 | FL022712 | TAC-operated | Group |
Collection:
Collection ID: | CO002034 |
Collection Summary: | Brown adipose tissue (BAT) was excised from the mice and immediately frozen in liquid nitrogen. |
Sample Type: | Adipose tissue |
Treatment:
Treatment ID: | TR002053 |
Treatment Summary: | All mice were randomly allocated for surgical procedures under blinded condition. Thoracic aortic constriction (TAC) was performed in 11-week-old male mice, and sample was collected 2 weeks after surgery. Sham-operated mice underwent the same procedures, except for aortic constriction. |
Sample Preparation:
Sampleprep ID: | SP002047 |
Sampleprep Summary: | To extract metabolites, 40~50mg snap-frozen samples were completely homogenized by Shake Master NEO (Bio Medical Science) in 500uL of methanol containing L-methionine sulfone (Wako 502-76641), methionine sulfone (MES, Dojindo 349-01623), and D-camphor-10-sulfonic acid sodium salt (CSA, Wako 037-01032) (all at 20 μM). After adding CHCl3 (500 μl) and distilled water (200 μl) with thorough mixing, centrifugation was performed at 4,600g for 15 min at 4°C. Then the aqueous layer (300 μl) was collected and transferred to a 5-kDa cutoff filter (UltrafreeMC-PLHCC; Human Metabolome Technologies, UFC3LCCNB-HMT). Centrifugation was performed at 9,100g for 3 hours at 20°C, after which the filtrate was centrifugally concentrated and dissolved in 50μl distilled water containing reference compounds (200 μM each of 3-aminopyrrolidine and trimesate). |
Combined analysis:
Analysis ID | AN003196 | AN003197 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | None (Direct infusion) | None (Direct infusion) |
Chromatography system | none | none |
Column | none | none |
MS Type | ESI | ESI |
MS instrument type | Single quadrupole | Single quadrupole |
MS instrument name | Agilent 6224 TOF | Agilent 6224 TOF |
Ion Mode | NEGATIVE | POSITIVE |
Units | nmol/g | nmol/g |
Chromatography:
Chromatography ID: | CH002364 |
Instrument Name: | none |
Column Name: | none |
Chromatography Type: | None (Direct infusion) |
MS:
MS ID: | MS002974 |
Analysis ID: | AN003196 |
Instrument Name: | Agilent 6224 TOF |
Instrument Type: | Single quadrupole |
MS Type: | ESI |
MS Comments: | Automatic recalibration of each acquired spectrum was performed using reference masses of reference standards ([13C isotopic ion of deprotonated acetic acid dimer (2CH3COOH - H)]-, m/z 120.03841), and ([Hexakis + deprotonated acetic acid (CH3COOH - H)]-, m/z 680.03554). Exact mass data were acquired at a rate of 1.5 spectra/s over a 50-1000 m/z range. Raw CE-TOFMS data were processed using Master Hands ver. 2.17.0.8 software for the quantification of metabolites. |
Ion Mode: | NEGATIVE |
MS ID: | MS002975 |
Analysis ID: | AN003197 |
Instrument Name: | Agilent 6224 TOF |
Instrument Type: | Single quadrupole |
MS Type: | ESI |
MS Comments: | Automatic recalibration of each acquired spectrum was performed using reference masses of reference standards ([2MeOH13Cisotope]-, m/z 66.06306), and ([Hexakis(2,2-difluoroethoxy)phosphazene]-, m/z 622.028963). Exact mass data were acquired at the rate of 1.5 cycles/s over a 50 to 1,000 m/z range. Raw CE-TOFMS data were processed using Master Hands ver. 2.17.0.8 software for the quantification of metabolites. |
Ion Mode: | POSITIVE |