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MB Sample ID: SA184818

Local Sample ID:T1_1_Al_exp3
Subject ID:SU002043
Subject Type:Cultured cells
Subject Species:Homo sapiens
Taxonomy ID:9606

Select appropriate tab below to view additional metadata details:

Subject:

Subject ID:SU002043
Subject Type:Cultured cells
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
T1_1_Al_exp3SA184818FL022722T1_1_AlCondition

Collection:

Collection ID:CO002036
Collection Summary:Lipids were extracted monophasic extractions of a mixture 1:1 of butanol-methanol (BuMe)
Sample Type:LnCap cells

Treatment:

Treatment ID:TR002055
Treatment Summary:These cells are CRISPR KOs of THEM6.

Sample Preparation:

Sampleprep ID:SP002049
Sampleprep Summary:Monophasic extraction straight from cell plates using BuMe or IPA.

Combined analysis:

Analysis ID AN003200 AN003201
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000
Column Waters Acquity CSH C18 (100 x 2.1mm,1.7um) Waters Acquity CSH C18 (100 x 2.1mm,1.7um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units Peak Area Peak area

Chromatography:

Chromatography ID:CH002366
Chromatography Summary:Please see methods
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Waters Acquity CSH C18 (100 x 2.1mm,1.7um)
Chromatography Type:Reversed phase

MS:

MS ID:MS002978
Analysis ID:AN003200
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:See Methods file.
Ion Mode:POSITIVE
  
MS ID:MS002979
Analysis ID:AN003201
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:See Methods file.
Ion Mode:NEGATIVE
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