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MB Sample ID: SA185583

Local Sample ID:	BM71P2
Subject ID:	SU002062
Subject Type:	Cultured cells
Subject Species:	Homo sapiens
Taxonomy ID:	9606

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Subject:

Subject ID:	SU002062
Subject Type:	Cultured cells
Subject Species:	Homo sapiens
Taxonomy ID:	9606

Factors:

Local Sample ID	MB Sample ID	Factor Level ID	Level Value	Factor Name
BM71P2	SA185583	FL022937	P2	experimental factor

Collection:

Collection ID:	CO002055
Collection Summary:	Each cell line has 10 replicates. Two bone marrow-derived MSC lines (RoosterBio, Frederick MD) lot #0071 (F, 18-30) and #0182 (F, 26) (which the manufacturer has both research and clinical-grade lots available), and one induced pluripotent stem cell derived MSC cell-line (Cellular Dynamics International, Madison WI) (Lot #0003, also prequalified) were used and referred here as BM71, BM182, and iMSC, respectively. iMSC were cultured for 25 days, BM71 were cultured for 21 days and BM182 were cultured for 23 days. 100 µl media was sampled daily from each sample and stored at -80◦C for further analysis.
Sample Type:	Bone marrow

Treatment:

Treatment ID:	TR002074
Treatment Summary:	Each cell line has 10 replicates. Two bone marrow-derived MSC lines (RoosterBio, Frederick MD) lot #0071 (F, 18-30) and #0182 (F, 26) (which the manufacturer has both research and clinical-grade lots available), and one induced pluripotent stem cell derived MSC cell-line (Cellular Dynamics International, Madison WI) (Lot #0003, also prequalified) were used and referred here as BM71, BM182, and iMSC, respectively. iMSC were cultured for 25 days, BM71 were cultured for 21 days and BM182 were cultured for 23 days. 100 µl media was sampled daily from each sample and stored at -80◦C for further analysis.

Treatment ID:

TR002074

Treatment Summary:

Each cell line has 10 replicates. Two bone marrow-derived MSC lines (RoosterBio, Frederick MD) lot #0071 (F, 18-30) and #0182 (F, 26) (which the manufacturer has both research and clinical-grade lots available), and one induced pluripotent stem cell derived MSC cell-line (Cellular Dynamics International, Madison WI) (Lot #0003, also prequalified) were used and referred here as BM71, BM182, and iMSC, respectively. iMSC were cultured for 25 days, BM71 were cultured for 21 days and BM182 were cultured for 23 days. 100 µl media was sampled daily from each sample and stored at -80◦C for further analysis.

Sample Preparation:

Sampleprep ID:	SP002068
Sampleprep Summary:	One hundred µl culture media was thawed on ice and then centrifuged at 14,000 x g for 15 min at 4◦C. For each sample, 54 µl of media supernatant was transferred to a new Eppendorf tube. 30 µl of remaining media in each sample from the same cell line was pooled together to generate six 54 µl internal quality control (QC) samples. 6 µl of 10/3 mM DSS-D6 (Cambridge Isotope Laboratory) in D2O (Cambridge Isotope Laboratory) was then added into each sample. In addition, six buffer blanks (6 µl of 10/3 mM DSS-D6 in 54 µl D2O) samples were added in each cell line for quality assurance purpose. We had 10 replicates for each cell line and time point. A total of 262 samples including 250 experimental samples, 6 pooled samples and 6 buffer blanks were generated for the iMSC group. A total of 222 samples including 210 experimental samples, 6 pooled samples and 6 buffer blanks were generated for BM71. A total of 242 samples including 230 experimental samples, 6 pooled samples and 6 buffer blanks were generated for BM182. All samples were randomized to reduce technical variance.

Sampleprep ID:

SP002068

Sampleprep Summary:

One hundred µl culture media was thawed on ice and then centrifuged at 14,000 x g for 15 min at 4◦C. For each sample, 54 µl of media supernatant was transferred to a new Eppendorf tube. 30 µl of remaining media in each sample from the same cell line was pooled together to generate six 54 µl internal quality control (QC) samples. 6 µl of 10/3 mM DSS-D6 (Cambridge Isotope Laboratory) in D2O (Cambridge Isotope Laboratory) was then added into each sample. In addition, six buffer blanks (6 µl of 10/3 mM DSS-D6 in 54 µl D2O) samples were added in each cell line for quality assurance purpose. We had 10 replicates for each cell line and time point. A total of 262 samples including 250 experimental samples, 6 pooled samples and 6 buffer blanks were generated for the iMSC group. A total of 222 samples including 210 experimental samples, 6 pooled samples and 6 buffer blanks were generated for BM71. A total of 242 samples including 230 experimental samples, 6 pooled samples and 6 buffer blanks were generated for BM182. All samples were randomized to reduce technical variance.

Analysis:

MB Sample ID:	SA185583
Analysis ID:	AN003231
Analysis Type:	NMR
Num Factors:	6
Num Metabolites:	50
Units:	change rate

NMR:

NMR ID:	NM000222
Analysis ID:	AN003231
Instrument Name:	bruker800
Instrument Type:	FT-NMR
NMR Experiment Type:	1D-1H
Spectrometer Frequency:	800