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MB Sample ID: SA187481
| Local Sample ID: | AB037_020 |
| Subject ID: | SU002084 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002084 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| AB037_020 | SA187481 | FL023128 | case | treatment |
Collection:
| Collection ID: | CO002077 |
| Collection Summary: | Plasma was collected from case and control patients |
| Sample Type: | Blood (plasma) |
Treatment:
| Treatment ID: | TR002096 |
| Treatment Summary: | Case versus control |
Sample Preparation:
| Sampleprep ID: | SP002090 |
| Sampleprep Summary: | Extraction is carried out using a bi-phasic solvent system of cold methanol, methyl tert-butyl ether (MTBE), and water. In more detail, cold methanol (225 µL is added to a 5mg tissue sample aliquot, which is placed into a 1.5 mL Eppendorf tube. Then, 750 µL of cold MTBE is added, followed by vortexing for 10 s. and shaking for 6 min. at 4ºC. Phase separation is induced by adding 188 µL of mass spec-grade water. After vortexing for 20 s. the sample is centrifuged at 14,000 rpm for 2 min. The upper organic phase is collected in two 300 µL aliquots for lipid analysis polar layer is collected in two 125 µL aliquots for HILIC analysis. One is stored at -20ºC as a backup and the other is evaporated to dryness in a SpeedVac. Dried extracts are resuspended in acetonitrile. |
Combined analysis:
| Analysis ID | AN003266 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Agilent 6600 |
| Column | Waters Acquity UPLC BEH amide,(150 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | TTOF |
| MS instrument name | Agilent 6560 Ion Mobility |
| Ion Mode | POSITIVE |
| Units | normalized peak height |
Chromatography:
| Chromatography ID: | CH002410 |
| Chromatography Summary: | The LC/QTOFMS analyses are performed using an Agilent 1290 Infinity LC system (G4220A binary pump, G4226A autosampler, and G1316C Column Thermostat) coupled to a SCIEX Triple TOF mass spectrometer. Polar compounds are separated on an Acquity UPLC BEH Amide Column, 130Å, 1.7 µm, 2.1 mm X 150 mm maintained at 45°C at a flow-rate of 0.4 mL/min. Solvent pre-heating (Agilent G1316) was used. The mobile phases consist of: Water, 10 mM Ammonium Formate, 0.125% Formic Acid (A) and Acetonitrile: Water (95/5, v/v), 10 mM Ammonium Formate, 0.125% Formic Acid (B) |
| Instrument Name: | Agilent 6600 |
| Column Name: | Waters Acquity UPLC BEH amide,(150 x 2.1mm,1.7um) |
| Column Temperature: | 45 |
| Flow Rate: | 0.4 mL/min |
| Solvent A: | 100% water; 0.125% formic acid; 10 mM ammonium formate |
| Solvent B: | 95% acetonitrile/5% water; 0.125% formic acid; 10 mM ammonium formate |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS003038 |
| Analysis ID: | AN003266 |
| Instrument Name: | Agilent 6560 Ion Mobility |
| Instrument Type: | TTOF |
| MS Type: | ESI |
| MS Comments: | SCIEX Triple TOF 6600mass spectrometers are operated with electrospray ionization (ESI) performing full scan in the mass range m/z 50–1200. Number of cycles in MS1 is 1667 with cycle time of 500ms and accumulation time 475ms. Mass spectrometer parameters are as follows (positive mode) Gas Temp 300°C, gas pressures in psi units with : GS1 and GS2 50 psi, CUR: 35. ISVF is 4500V and DP and CE are 10V and 100 V. |
| Ion Mode: | POSITIVE |
