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MB Sample ID: SA193710
| Local Sample ID: | 2021_06_16_Jorida_Run 02 |
| Subject ID: | SU002136 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002136 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 2021_06_16_Jorida_Run 02 | SA193710 | FL023726 | CHLA 136 | Genotype |
Collection:
| Collection ID: | CO002129 |
| Collection Summary: | Cells were collected in cold PBS and suspended in cold Buffer A with protease inhibitor at 10 million cells/ml |
| Sample Type: | Cultured cells |
Treatment:
| Treatment ID: | TR002148 |
| Treatment Summary: | Cells were treated with IMDM Media 12440 supplemented in 10% fetal bovine serum, 2mM L-glutamine, 1% ITS, 100U/ml of penicillin and 100 mcg/ml gentamycin. Cell cultures were incubated at 37 degree Celsius in a humidified atmosphere of 5% CO2. |
Sample Preparation:
| Sampleprep ID: | SP002142 |
| Sampleprep Summary: | Lipids were extracted from the cell pellet (Bligh/Dyer). 900 ul chlorofrom/methanol (1/2) into the cell pellet, then incubated at 4C for 1 hour agigated (300rpm). Chloroform(300ul) and MQ water(250ul) were added discrete order, then vortex and cetrifuged for 2min at 9000 rpm. Bottom organic phase was collected and dried using vacuum concentrator. |
Combined analysis:
| Analysis ID | AN003344 | AN003345 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Waters Acquity I-Class | Waters Acquity I-Class |
| Column | Waters Acquity BEH HSS T3 (150x 2.1mm,1.8um) | Waters Acquity BEH HSS T3 (150x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Waters Synapt G2 Si QTOF | Waters Synapt G2 Si QTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | uM | uM |
Chromatography:
| Chromatography ID: | CH002476 |
| Instrument Name: | Waters Acquity I-Class |
| Column Name: | Waters Acquity BEH HSS T3 (150x 2.1mm,1.8um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS003113 |
| Analysis ID: | AN003344 |
| Instrument Name: | Waters Synapt G2 Si QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Raw data were converted into ABF files using Analysis Base File Converter. These converted files were used for MS-Dial to align/identify peaks/calculate peak area. |
| Ion Mode: | POSITIVE |
| MS ID: | MS003114 |
| Analysis ID: | AN003345 |
| Instrument Name: | Waters Synapt G2 Si QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Raw data were converted into ABF files using Analysis Base File Converter. These converted files were used for MS-Dial to align/identify peaks/calculate peak area. |
| Ion Mode: | NEGATIVE |
