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MB Sample ID: SA193720
Local Sample ID: | 2021_06_16_Jorida_Run 03 |
Subject ID: | SU002136 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002136 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
2021_06_16_Jorida_Run 03 | SA193720 | FL023729 | COGN 144 | Genotype |
Collection:
Collection ID: | CO002129 |
Collection Summary: | Cells were collected in cold PBS and suspended in cold Buffer A with protease inhibitor at 10 million cells/ml |
Sample Type: | Cultured cells |
Treatment:
Treatment ID: | TR002148 |
Treatment Summary: | Cells were treated with IMDM Media 12440 supplemented in 10% fetal bovine serum, 2mM L-glutamine, 1% ITS, 100U/ml of penicillin and 100 mcg/ml gentamycin. Cell cultures were incubated at 37 degree Celsius in a humidified atmosphere of 5% CO2. |
Sample Preparation:
Sampleprep ID: | SP002142 |
Sampleprep Summary: | Lipids were extracted from the cell pellet (Bligh/Dyer). 900 ul chlorofrom/methanol (1/2) into the cell pellet, then incubated at 4C for 1 hour agigated (300rpm). Chloroform(300ul) and MQ water(250ul) were added discrete order, then vortex and cetrifuged for 2min at 9000 rpm. Bottom organic phase was collected and dried using vacuum concentrator. |
Combined analysis:
Analysis ID | AN003344 | AN003345 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Waters Acquity I-Class | Waters Acquity I-Class |
Column | Waters Acquity BEH HSS T3 (150x 2.1mm,1.8um) | Waters Acquity BEH HSS T3 (150x 2.1mm,1.8um) |
MS Type | ESI | ESI |
MS instrument type | QTOF | QTOF |
MS instrument name | Waters Synapt G2 Si QTOF | Waters Synapt G2 Si QTOF |
Ion Mode | POSITIVE | NEGATIVE |
Units | uM | uM |
Chromatography:
Chromatography ID: | CH002476 |
Instrument Name: | Waters Acquity I-Class |
Column Name: | Waters Acquity BEH HSS T3 (150x 2.1mm,1.8um) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS003113 |
Analysis ID: | AN003344 |
Instrument Name: | Waters Synapt G2 Si QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | Raw data were converted into ABF files using Analysis Base File Converter. These converted files were used for MS-Dial to align/identify peaks/calculate peak area. |
Ion Mode: | POSITIVE |
MS ID: | MS003114 |
Analysis ID: | AN003345 |
Instrument Name: | Waters Synapt G2 Si QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | Raw data were converted into ABF files using Analysis Base File Converter. These converted files were used for MS-Dial to align/identify peaks/calculate peak area. |
Ion Mode: | NEGATIVE |