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MB Sample ID: SA193878
| Local Sample ID: | NM_JHY_S13 |
| Subject ID: | SU002139 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002139 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male and female |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| NM_JHY_S13 | SA193878 | FL023740 | Untreated Control | Treatment |
Collection:
| Collection ID: | CO002132 |
| Collection Summary: | Primary Human Hepatocytes provided by BioIVT. Collected from deceased subjects |
| Sample Type: | Cultured cells |
Treatment:
| Treatment ID: | TR002151 |
| Treatment Summary: | NASH hepatocytes were taken from patients with NASH. IVIS Hepatocytes were cultured and treated with fatty acid enriched media |
| Treatment Compound: | Free fatty acids |
| Cell Growth Config: | 24-well plate |
Sample Preparation:
| Sampleprep ID: | SP002145 |
| Sampleprep Summary: | Lipidomics extraction using C:W:M extraction followed by ButOH:MeOH of residual culture plate. Extractions were dried down, combined, and reconstituted. |
| Processing Storage Conditions: | -80℃ |
| Extract Storage: | -80℃ |
Combined analysis:
| Analysis ID | AN003349 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 |
| Column | Ascentis Express C8,(2.7um 100mm) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | UNSPECIFIED |
| Units | AU |
Chromatography:
| Chromatography ID: | CH002480 |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Ascentis Express C8,(2.7um 100mm) |
| Column Temperature: | 40 |
| Flow Rate: | 0.2 mL/min |
| Sample Injection: | 10 uL |
| Solvent A: | 60% water/40% isopropanol; 2 mM formic acid; 8 mM ammonium carbonate |
| Solvent B: | 98% isopropanol/2% water; 2 mM formic acid; 8 mM ammonium carbonate |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS003118 |
| Analysis ID: | AN003349 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | The LC-MS raw file conversion was performed using ProteoWizard and XCMS. Mzmatch.R was then used for alignment and filtering of peaks with an intensity cut-off of 100,000, relative standard deviation of less than 0.8, and peak shape noise filter of greater than 0.8. Output was then further processed using IDEOM |
| Ion Mode: | UNSPECIFIED |
| Ion Spray Voltage: | 3.5 kV |
