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MB Sample ID: SA194082
Local Sample ID: | 203-10336_CON-IL_0.raw |
Subject ID: | SU002145 |
Subject Type: | Mammal |
Subject Species: | Bos taurus |
Taxonomy ID: | 9913 |
Genotype Strain: | Holstein dairy cows |
Age Or Age Range: | 4- 5 years |
Weight Or Weight Range: | 733-797 |
Gender: | Female |
Animal Housing: | Tie stall |
Animal Feed: | TMR Ad lib |
Animal Water: | Ad libitum |
Animal Inclusion Criteria: | lactation stage, Days in milk |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002145 |
Subject Type: | Mammal |
Subject Species: | Bos taurus |
Taxonomy ID: | 9913 |
Genotype Strain: | Holstein dairy cows |
Age Or Age Range: | 4- 5 years |
Weight Or Weight Range: | 733-797 |
Gender: | Female |
Animal Housing: | Tie stall |
Animal Feed: | TMR Ad lib |
Animal Water: | Ad libitum |
Animal Inclusion Criteria: | lactation stage, Days in milk |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
203-10336_CON-IL_0.raw | SA194082 | FL023777 | - | Time |
203-10336_CON-IL_0.raw | SA194082 | FL023777 | CON | Treatment |
203-10336_CON-IL_0.raw | SA194082 | FL023777 | Lipidomics | Category |
Collection:
Collection ID: | CO002138 |
Collection Summary: | Blood was collected via coccygeal venipuncture after the morning milking during the baseline period and at -0.5, 0, 4, 8, 16, 24, and 48 h, relative to saline or LPS administration. Plasma samples were incubated on ice for 30 min before centrifugation. |
Collection Protocol ID: | N/A |
Collection Protocol Filename: | Collection_Protocol.docx |
Collection Protocol Comments: | N/A |
Sample Type: | Blood (plasma) |
Collection Method: | Blood collection using venipuncture needle |
Collection Location: | Coccygeal venipuncture |
Collection Frequency: | At interval of -0.5, 0, 4, 8, 16, 24, and 48 h respectively |
Collection Duration: | 1 min |
Volumeoramount Collected: | 10ml |
Storage Conditions: | -20℃ |
Collection Vials: | 10 ml blood vial |
Storage Vials: | Eppendorf tubes 2ml |
Collection Tube Temp: | 4°C |
Treatment:
Treatment ID: | TR002157 |
Treatment Summary: | Sterile saline (CON; n = 5) or 2) LPS (0.375 μg of LPS/kg of BW in sterile saline; Escherichia coli O55:B5; Sigma Aldrich, St. Louis, MO; n = 5) |
Treatment Protocol ID: | N/A |
Treatment Protocol Filename: | N A |
Sample Preparation:
Sampleprep ID: | SP002151 |
Sampleprep Summary: | Untargeted lipidomics samples were prepared by dissolving in dicholormethane/ methanol ( MeOH; 2:1). Untargeted metabolomics was done by centrifugation, samples were re-suspended with 60% ACN prior to analysis. FOR details please refer to publication Wang et al 2022. |
Combined analysis:
Analysis ID | AN003362 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Vanquish UHPLC system |
Column | Accucore C30 (150 x 2.1mm,2.6um) |
MS Type | ESI |
MS instrument type | Single quadrupole |
MS instrument name | Thermo Q Exactive HF hybrid Orbitrap |
Ion Mode | UNSPECIFIED |
Units | Normalized ion intensity |
Chromatography:
Chromatography ID: | CH002488 |
Chromatography Summary: | Chromatographic separation was performed on a Vanquish UHPLC system with an Accucore C30, 2.6 μm column (2.1 mm id × 150mm) coupled to a Q Exactive™ Hybrid Quadrupole-Orbitrap High Resolution Mass Spectrometer (Thermo Fisher Scientific, San Jose, CA) and generated data was processed using LipidSearch™ software version 4.1 (Thermo Scientific), |
Methods Filename: | Genenral_method_for_Untargeted_Metabolomics_Mar2020.docx |
Instrument Name: | Vanquish UHPLC system |
Column Name: | Accucore C30 (150 x 2.1mm,2.6um) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS003131 |
Analysis ID: | AN003362 |
Instrument Name: | Thermo Q Exactive HF hybrid Orbitrap |
Instrument Type: | Single quadrupole |
MS Type: | ESI |
MS Comments: | Please refer to publication Wang et al 2022. |
Ion Mode: | UNSPECIFIED |