Return to study ST002070 main page
MB Sample ID: SA194769
| Local Sample ID: | 49 QC40 |
| Subject ID: | SU002152 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male |
| Cell Strain Details: | HCT 116 |
| Subject Comments: | HCT 116 cell line was cultured as a 2D monolayer and 3D spheroids |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002152 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male |
| Cell Strain Details: | HCT 116 |
| Subject Comments: | HCT 116 cell line was cultured as a 2D monolayer and 3D spheroids |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 49 QC40 | SA194769 | FL023902 | QC8 | GROUP |
| 49 QC40 | SA194769 | FL023902 | QC | TYPE |
Collection:
| Collection ID: | CO002145 |
| Collection Summary: | Cell Lysates from 2D Monolayers and serially-trypsinized spheroids was subjected to lipid extraction |
| Sample Type: | Cultured cells |
| Storage Conditions: | -80℃ |
| Collection Tube Temp: | 4 |
| Additives: | 1X PBS |
Treatment:
| Treatment ID: | TR002164 |
| Treatment Summary: | HCT 116 2D monolayers were cultured per ATCC protocol, while HCT 116 3D spheroids were cultured for 14 days. At Day 14, the spheroids were subjected to Serial Trypsinization in order to obtain discrete cell populations from spheroids representing the outer, middle, and core regions. |
Sample Preparation:
| Sampleprep ID: | SP002158 |
| Sampleprep Summary: | Lipid extraction by MTBE/methanol/water solvent system |
| Processing Storage Conditions: | On ice |
| Extraction Method: | liquid-liquid extraction |
| Extract Storage: | -80℃ |
| Sample Resuspension: | 9:1 Methanol/Toluene |
| Sample Spiking: | equiSPLASH LIPIDOMIX standards |
Combined analysis:
| Analysis ID | AN003374 | AN003375 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Agilent 1260 | Agilent 1260 |
| Column | Thermo Accucore C30 (100 x 2.1mm,2.6um) | Thermo Accucore C30 (100 x 2.1mm,2.6um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Agilent 6545 QTOF | Agilent 6545 QTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | pmol lipid/ ug protein | pmol lipid/ ug protein |
Chromatography:
| Chromatography ID: | CH002494 |
| Instrument Name: | Agilent 1260 |
| Column Name: | Thermo Accucore C30 (100 x 2.1mm,2.6um) |
| Column Temperature: | 50 |
| Flow Rate: | 400uL/min |
| Injection Temperature: | 4 |
| Internal Standard: | equiSPLASH LIPIDOMIX |
| Sample Injection: | 2 |
| Solvent A: | 50% water/50% acetonitrile; 10mM ammonium formate |
| Solvent B: | 90% isopropanol/10% acetonitrile; 10 mM ammonium formate |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS003141 |
| Analysis ID: | AN003374 |
| Instrument Name: | Agilent 6545 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | MS-Only on biological samples, Auto-MSMS on Pooled Sample |
| Ion Mode: | POSITIVE |
| MS ID: | MS003142 |
| Analysis ID: | AN003375 |
| Instrument Name: | Agilent 6545 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | MS-Only on biological samples, Auto-MSMS on Pooled Sample |
| Ion Mode: | NEGATIVE |
