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MB Sample ID: SA194783
| Local Sample ID: | adenoma_dan_HCl_2 |
| Subject ID: | SU002154 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002154 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| adenoma_dan_HCl_2 | SA194783 | FL023906 | adenoma | Treatment |
Collection:
| Collection ID: | CO002147 |
| Collection Summary: | As stated in the paper: Colorectal tissue was stored at -80 °C until processing. Cryosections of the colorectal tissue, containing the adenoma, were taken at 10 µm thickness and were mounted on indium tin oxide (ITO) slides for MALDI MSI analysis. Serial sections were obtained for MALDI MSI and immunofluorescence microscopy using a pPDH antibody and DAPI staining. The cryosections used for immunofluorescence were 5 µm in thickness. Fluorescent microscopy images were acquired using a 40x objective (Zeiss Observer Z.1, Oberkochen, Germany), a DAPI filter (Filter Set 49, Carl Zeiss Microscopy, Oberkochen, Germany), and an FITC filter (31001, Chroma Technology Corporation, Bellows Falls, VT). |
| Sample Type: | Colon |
Treatment:
| Treatment ID: | TR002166 |
| Treatment Summary: | N/A |
Sample Preparation:
| Sampleprep ID: | SP002160 |
| Sampleprep Summary: | As stated in the paper: 4.4 mg/mL of 1,5-diaminonapthalene hydrochloride (CAS: 2243-62-41, Sigma-Aldrich, Darnstadt, Germany) was dissolved in 4/4.5/0.5 HPLC grade water/ethanol/1 M HCl (v/v/v). 28 The 10 µm thick tissue sections were sprayed using a TM-sprayer (HTX Technologies, Chapel Hill, NC) in a four-pass method. The parameters of the matrix application set in the TM-sprayer were as follows: spray nozzle velocity (1200 mm/min), track spacing (2 mm), flow rate (0.09 mL/min), spray nozzle temperature (75 °C), and nitrogen gas pressure (10 psi) |
Combined analysis:
| Analysis ID | AN003377 |
|---|---|
| Analysis type | MS |
| Chromatography type | None (Direct infusion) |
| Chromatography system | timsTOF fleX |
| Column | none |
| MS Type | MALDI |
| MS instrument type | QTOF |
| MS instrument name | Bruker timsTOF fleX |
| Ion Mode | NEGATIVE |
| Units | Da |
Chromatography:
| Chromatography ID: | CH002496 |
| Instrument Name: | timsTOF fleX |
| Column Name: | none |
| Chromatography Type: | None (Direct infusion) |
MS:
| MS ID: | MS003144 |
| Analysis ID: | AN003377 |
| Instrument Name: | Bruker timsTOF fleX |
| Instrument Type: | QTOF |
| MS Type: | MALDI |
| MS Comments: | SCilS 2022a pro |
| Ion Mode: | NEGATIVE |
