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MB Sample ID: SA196391

Local Sample ID:TCMDC-124553-3
Subject ID:SU002161
Subject Type:Cultured cells
Subject Species:Plasmodium falciparum
Taxonomy ID:5833
Genotype Strain:3D7

Select appropriate tab below to view additional metadata details:

Subject:

Subject ID:SU002161
Subject Type:Cultured cells
Subject Species:Plasmodium falciparum
Taxonomy ID:5833
Genotype Strain:3D7

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
TCMDC-124553-3SA196391FL023954TCMDC-124553Drug

Collection:

Collection ID:CO002154
Collection Summary:Cells were pelleted and the media aspirated prior to extraction
Sample Type:Pf infected RBCs

Treatment:

Treatment ID:TR002173
Treatment Summary:Purified iRBCs were treated with 10XIC50 drug for 2.5 hours

Sample Preparation:

Sampleprep ID:SP002167
Sampleprep Summary:90% Methanol/10% water at 50:1 solvent/cell pellet followed by vortexing and clarification via centrifugation
Processing Method:Lysis
Processing Storage Conditions:4℃
Extraction Method:See SA Cobbold et al JBC 2013
Extract Cleanup:Clarification via centrifugation followed by nitrogen drying
Extract Storage:-80℃
Sample Spiking:Internal Standard 13C4, 15N1-Aspartate used for sample preparation normalizer

Combined analysis:

Analysis ID AN003387 AN003388 AN003389 AN003390
Analysis type MS MS MS MS
Chromatography type Reversed phase Reversed phase Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 RS Thermo Dionex Ultimate 3000 RS Thermo Dionex Ultimate 3000 RS Thermo Dionex Ultimate 3000 RS
Column Waters XSelect HSS T3 C18 (100 x 2.1mm,2.5um) Water XSelect HSS T3 C18 (100 x 2.1mm,2.5um) Water XSelect HSS T3 C18 (100 x 2.1mm,2.5um) Water XSelect HSS T3 C18 (100 x 2.1mm,2.5um)
MS Type ESI ESI ESI ESI
MS instrument type Orbitrap Orbitrap Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Plus Orbitrap Thermo Q Exactive Plus Orbitrap Thermo Q Exactive Plus Orbitrap Thermo Q Exactive Plus Orbitrap
Ion Mode NEGATIVE NEGATIVE NEGATIVE NEGATIVE
Units peak area peak area peak area peak area

Chromatography:

Chromatography ID:CH002503
Instrument Name:Thermo Dionex Ultimate 3000 RS
Column Name:Waters XSelect HSS T3 C18 (100 x 2.1mm,2.5um)
Internal Standard:13C4, 15N1-Aspartate
Preconditioning:Water Blanks
Chromatography Type:Reversed phase
  
Chromatography ID:CH002504
Instrument Name:Thermo Dionex Ultimate 3000 RS
Column Name:Water XSelect HSS T3 C18 (100 x 2.1mm,2.5um)
Internal Standard:13C4, 15N1-Aspartate
Preconditioning:Water Blanks
Chromatography Type:Reversed phase
  
Chromatography ID:CH002505
Instrument Name:Thermo Dionex Ultimate 3000 RS
Column Name:Water XSelect HSS T3 C18 (100 x 2.1mm,2.5um)
Internal Standard:13C4, 15N1-Aspartate
Preconditioning:Water Blanks
Chromatography Type:Reversed phase
  
Chromatography ID:CH002506
Instrument Name:Thermo Dionex Ultimate 3000 RS
Column Name:Water XSelect HSS T3 C18 (100 x 2.1mm,2.5um)
Internal Standard:13C4, 15N1-Aspartate
Preconditioning:Water Blanks
Chromatography Type:Reversed phase

MS:

MS ID:MS003154
Analysis ID:AN003387
Instrument Name:Thermo Q Exactive Plus Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:El-Maven was used to process converted .mzXML
Ion Mode:NEGATIVE
  
MS ID:MS003155
Analysis ID:AN003388
Instrument Name:Thermo Q Exactive Plus Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:El-Maven was used to process converted .mzXML
Ion Mode:NEGATIVE
  
MS ID:MS003156
Analysis ID:AN003389
Instrument Name:Thermo Q Exactive Plus Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:El-Maven was used to process converted .mzXML
Ion Mode:NEGATIVE
  
MS ID:MS003157
Analysis ID:AN003390
Instrument Name:Thermo Q Exactive Plus Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:El-Maven was used to process converted .mzXML
Ion Mode:NEGATIVE
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