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MB Sample ID: SA196470
| Local Sample ID: | sham_11 |
| Subject ID: | SU002163 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002163 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| sham_11 | SA196470 | FL023968 | 1-month | time point |
Collection:
| Collection ID: | CO002156 |
| Collection Summary: | Plasma was obtained from the blood samples upon centrifugation and stored at −80 °C until analysis. |
| Sample Type: | Blood (plasma) |
Treatment:
| Treatment ID: | TR002175 |
| Treatment Summary: | The mouse model was induced via MCAO using a 6-0 nylon monolayer suture. Animals were anesthetized with 2% isoflurane in 30% oxygen and 70% nitrous oxide using a face mask. The mice were anesthetized, and blood was collected by retro-orbital bleeding using sterile capillary tubes. |
Sample Preparation:
| Sampleprep ID: | SP002169 |
| Sampleprep Summary: | plasma samples were processed through a simple protein precipitation technique using 150 µL of ice-cold methanol using plasma methanol ratio of 1:3. After the addition of methanol, the mixture was then vortexed and centrifuged at 20,800×g for 10 min at 4 °C. |
Combined analysis:
| Analysis ID | AN003393 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Orbitrap Velos Pro mass spectrometer system |
| Column | Waters ACQUITY UPLC BEH C18 |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Velos LTQ Orbitrap |
| Ion Mode | NEGATIVE |
| Units | peak area |
Chromatography:
| Chromatography ID: | CH002508 |
| Instrument Name: | Orbitrap Velos Pro mass spectrometer system |
| Column Name: | Waters ACQUITY UPLC BEH C18 |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS003160 |
| Analysis ID: | AN003393 |
| Instrument Name: | Thermo Velos LTQ Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | - |
| Ion Mode: | NEGATIVE |
