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MB Sample ID: SA198400
| Local Sample ID: | 41-Serum_CG_T1M |
| Subject ID: | SU002170 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 24-65 |
| Gender: | Male and female |
| Human Ethnicity: | Caucasian |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002170 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 24-65 |
| Gender: | Male and female |
| Human Ethnicity: | Caucasian |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 41-Serum_CG_T1M | SA198400 | FL024112 | Yes | Previus_COVID |
| 41-Serum_CG_T1M | SA198400 | FL024112 | T1M | TIME |
Collection:
| Collection ID: | CO002163 |
| Collection Summary: | For all subjects blood serum samples were collected at six different time points: before the first dose (T0); 7 and 14 days after the first dose (T7 and T14, respectively); 21 days after the first dose, just before the second dose (T21); 28 days after the first dose and 7 days after the second dose (T28); 1 month after the second dose (T1M). Blood samples were collected (4 hours after breakfast) in a BD vacutainer clot-activator tube for serum collection and processed within one hour from sample collection. After processing, all the serum samples were immediately stored at -30°C until NMR analysis. |
| Sample Type: | Blood (serum) |
| Storage Conditions: | Described in summary |
| Collection Vials: | BD vacutainer clot-activator tube for serum |
Treatment:
| Treatment ID: | TR002182 |
| Treatment Summary: | We monitor the effect of mRNA Pfizer-BioNTech vaccine in a cohort of 20 healthcare workers. All of them received two doses, 21 days apart. |
| Treatment: | Pfizer-BioNTech mRNA vaccine |
Sample Preparation:
| Sampleprep ID: | SP002176 |
| Sampleprep Summary: | NMR samples were prepared according to standard procedures. Frozen serum samples were thawed at room temperature. A total of 350 μL of sodium phosphate buffer (70 mM Na2HPO4; 20% (v/v) 2H2O; 6.1 mM NaN3, 4.6 mM sodium trimethylsilyl [2,2,3,3−2H4] propionate (TMSP), pH 7.4) was added to 350 μL of each serum sample; the mixture was homogenized by vortexing for 30 s. A total of 600 μL of each mixture was transferred into a 5.00 mm NMR tube (Bruker BioSpin) for the analysis. |
| Processing Storage Conditions: | -80℃ |
Analysis:
| MB Sample ID: | SA198400 |
| Analysis ID: | AN003404 |
| Analysis Type: | NMR |
| Num Factors: | 12 |
| Num Metabolites: | 114 |
| Units: | mM |
NMR:
| NMR ID: | NM000230 |
| Analysis ID: | AN003404 |
| Instrument Name: | Bruker |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D-1H |
| Spectrometer Frequency: | 600MHz |
| NMR Probe: | 5 mm PATXI 1H−13C−15N and 2H-decoupling probe |
| NMR Tube Size: | 5.00 mm |
| Pulse Sequence: | NOESY |
| Water Suppression: | 1Dpresat |
| Temperature: | 36,85 |
| Number Of Scans: | 32 |
| Dummy Scans: | 4 |
| Acquisition Time: | 2.7 s |
| Relaxation Delay: | 4 s |
| Spectral Width: | 18,028 Hz |
| Num Data Points Acquired: | 98,304 |
| Line Broadening: | 0.3 |
| Binned Increment: | 0.02 ppm |
| Binned Data Excluded Range: | 4.5-5.5 ppm |
| NMR Results File: | NOESY.txt UNITS:ppm |
