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MB Sample ID: SA198409
Local Sample ID: | 50-Serum_DD_T21 |
Subject ID: | SU002170 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | 24-65 |
Gender: | Male and female |
Human Ethnicity: | Caucasian |
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Subject:
Subject ID: | SU002170 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | 24-65 |
Gender: | Male and female |
Human Ethnicity: | Caucasian |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
50-Serum_DD_T21 | SA198409 | FL024113 | Yes | Previus_COVID |
50-Serum_DD_T21 | SA198409 | FL024113 | T21 | TIME |
Collection:
Collection ID: | CO002163 |
Collection Summary: | For all subjects blood serum samples were collected at six different time points: before the first dose (T0); 7 and 14 days after the first dose (T7 and T14, respectively); 21 days after the first dose, just before the second dose (T21); 28 days after the first dose and 7 days after the second dose (T28); 1 month after the second dose (T1M). Blood samples were collected (4 hours after breakfast) in a BD vacutainer clot-activator tube for serum collection and processed within one hour from sample collection. After processing, all the serum samples were immediately stored at -30°C until NMR analysis. |
Sample Type: | Blood (serum) |
Storage Conditions: | Described in summary |
Collection Vials: | BD vacutainer clot-activator tube for serum |
Treatment:
Treatment ID: | TR002182 |
Treatment Summary: | We monitor the effect of mRNA Pfizer-BioNTech vaccine in a cohort of 20 healthcare workers. All of them received two doses, 21 days apart. |
Treatment: | Pfizer-BioNTech mRNA vaccine |
Sample Preparation:
Sampleprep ID: | SP002176 |
Sampleprep Summary: | NMR samples were prepared according to standard procedures. Frozen serum samples were thawed at room temperature. A total of 350 μL of sodium phosphate buffer (70 mM Na2HPO4; 20% (v/v) 2H2O; 6.1 mM NaN3, 4.6 mM sodium trimethylsilyl [2,2,3,3−2H4] propionate (TMSP), pH 7.4) was added to 350 μL of each serum sample; the mixture was homogenized by vortexing for 30 s. A total of 600 μL of each mixture was transferred into a 5.00 mm NMR tube (Bruker BioSpin) for the analysis. |
Processing Storage Conditions: | -80℃ |
Analysis:
MB Sample ID: | SA198409 |
Analysis ID: | AN003404 |
Analysis Type: | NMR |
Num Factors: | 12 |
Num Metabolites: | 114 |
Units: | mM |
NMR:
NMR ID: | NM000230 |
Analysis ID: | AN003404 |
Instrument Name: | Bruker |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D-1H |
Spectrometer Frequency: | 600MHz |
NMR Probe: | 5 mm PATXI 1H−13C−15N and 2H-decoupling probe |
NMR Tube Size: | 5.00 mm |
Pulse Sequence: | NOESY |
Water Suppression: | 1Dpresat |
Temperature: | 36,85 |
Number Of Scans: | 32 |
Dummy Scans: | 4 |
Acquisition Time: | 2.7 s |
Relaxation Delay: | 4 s |
Spectral Width: | 18,028 Hz |
Num Data Points Acquired: | 98,304 |
Line Broadening: | 0.3 |
Binned Increment: | 0.02 ppm |
Binned Data Excluded Range: | 4.5-5.5 ppm |
NMR Results File: | NOESY.txt UNITS:ppm |