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MB Sample ID: SA201409
| Local Sample ID: | AL-3 |
| Subject ID: | SU002186 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Male |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002186 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Male |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| AL-3 | SA201409 | FL024500 | Only intraperitoneally (i.p) injected with 1% CCl4 (10 mL/kg, dissolved in olive oil) at the 12th injection. During the previous 11 times they were treated the same as the healthy controls with an equal volume of olive oil. | Treatment |
Collection:
| Collection ID: | CO002179 |
| Collection Summary: | Animals were killed at 24h post the last CCl4 treatment and the liver tissue samples were harvested for further assays. |
| Sample Type: | Liver |
Treatment:
| Treatment ID: | TR002198 |
| Treatment Summary: | Prior to experimental, animals were acclimatized breeding to laboratory conditions for 1 week. The mice were randomly allocated into 6 groups (n=8 each), as follows: a control group (C); two acute hepatitis groups, low and high concentrations (AL/ AH); three chronic hepatitis groups, low, middle and high concentration (CL/ CM/ CH). Mice in chronic groups were treated 3 times per week with 4 consecutive respectively intraperitoneal (i.p) injection with 1% / 5% / 10% CCl4 (10 mL/kg, dissolved in olive oil). However, in the acute groups, mice were only intraperitoneally (i.p) injected with 1% / 5% CCl4 (10 mL/kg, dissolved in olive oil) at the 12th injection. During the previous 11 times they were treated the same as the healthy controls with an equal volume of olive oil. Animals were killed at 24h post the last CCl4 treatment and serum, stool and liver tissue samples were harvested for further assays. |
Sample Preparation:
| Sampleprep ID: | SP002192 |
| Sampleprep Summary: | 200-220 mg liver tissues mixed with 1 mL 0.1×PBS were homogenized using the Mini-BeadBeater-16. 225 μL of methanol, 750 μL of MTBE and 188 μL of water were added into 20 μL of liver homogenate for lipid extraction. The supernatant was blown dry in nitrogen, reconstituted in 100 µL of organic solvent (IPA: ACN: water = 30: 65: 5, v/v/v) and transferred to auto-sampler vials. |
Combined analysis:
| Analysis ID | AN003434 | AN003435 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | SCIEX ExionLC AD | SCIEX ExionLC AD |
| Column | Phenomenex Kinetex C18(100 x 3.0mm,2.6m) | Phenomenex Kinetex C18(100 x 3.0mm,2.6m) |
| MS Type | ESI | ESI |
| MS instrument type | QTRAP | QTRAP |
| MS instrument name | ABI Sciex 6500 QTrap | ABI Sciex 6500 QTrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | counts | counts |
Chromatography:
| Chromatography ID: | CH002538 |
| Chromatography Summary: | The resuspended samples were placed on the sample-tray at 4 ℃ with 1 μL-volume injection for targeted-lipidomics analysis, which was performed on an UHPLC Q-Trap/MS system (Qtrap 6500, SCIEX, USA). A Kinetex C18 column (100 mm × 3.0 mm, 2.6 μm; Phenomenex); mobile phase A was methanol: ACN: water = 1:1:1 (v/v/v) with 5 mM ammonium acetate, while mobile phase B was IPA with 5 mM ammonium acetate. The following gradient-elution program was 0-0.5 min, 80% A; 0.5-1.5 min, 80%-60% A; 1.5-3 min, 60%-40% A; 3-13 min, 40%-2% A; 13-13.1 min, 2%-80% A; 13.1-17 min, 80% A. The flow rate was at 0.3 mL/min and the column temperature was maintained at 50 ℃. |
| Instrument Name: | SCIEX ExionLC AD |
| Column Name: | Phenomenex Kinetex C18(100 x 3.0mm,2.6m) |
| Column Temperature: | 50 |
| Flow Gradient: | 0-0.5 min, 80% A; 0.5-1.5 min, 80%-60% A; 1.5-3 min, 60%-40% A; 3-13 min, 40%-2% A; 13-13.1 min, 2%-80% A; 13.1-17 min, 80% A |
| Flow Rate: | 0.3 mL/min |
| Solvent A: | 33.3% methanol/33.3% acetonitrile/33.3% water; 5 mM ammonium acetate, 100% isopropanol; 5 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS003197 |
| Analysis ID: | AN003434 |
| Instrument Name: | ABI Sciex 6500 QTrap |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | Analyst® (version 1.7.1, AB Sciex, Monza, Italy) Multiquant™ (version 3.0.31721.0, AB Sciex, Monza, Italy) |
| Ion Mode: | POSITIVE |
| MS ID: | MS003198 |
| Analysis ID: | AN003435 |
| Instrument Name: | ABI Sciex 6500 QTrap |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | Analyst® (version 1.7.1, AB Sciex, Monza, Italy) Multiquant™ (version 3.0.31721.0, AB Sciex, Monza, Italy) |
| Ion Mode: | NEGATIVE |
