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MB Sample ID: SA202854
| Local Sample ID: | Con.20 |
| Subject ID: | SU002198 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 35-85 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002198 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 35-85 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Con.20 | SA202854 | FL024962 | - | AJCC_stage |
Collection:
| Collection ID: | CO002191 |
| Collection Summary: | Before the first-diagnosed patient is admitted to the hospital for treatment and before the blood is drawn from the healthy controls, all the subjects were fasted overnight. The blood samples were taken through a clinical standard procedure, and 3-5 mL of venous blood was collected in the early morning and placed at 4 ºC for 2 hours. The serum was separated through a centrifugation at 3000 g for 10 minutes, and 500 μL of the supernatant was transferred into a 1.5-mL EP tube. The serum samples were snap-frozen with liquid nitrogen to stop the metabolic activity and then kept at -80 ºC. |
| Collection Protocol Filename: | NMR_serum collection.docx |
| Sample Type: | Blood (serum) |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR002210 |
| Treatment Summary: | None |
Sample Preparation:
| Sampleprep ID: | SP002204 |
| Sampleprep Summary: | A serum sample was prepared by mixing of 400 μL of serum with 200 μL of deuterated phosphate buffer solution (90 mM, pH=7.4) containing 0.9% NaCl (normal saline). The sample was mixed well by an oscillator and remained for 5 min. After a centrifugation for 10 min at 10,000 g and 4 ºC, 550 μL of the supernatant was transferred into a 5-mm NMR tube (ST500, NORELL, Inc., Morganton, North Carolina, USA) for NMR sampling. |
| Sampleprep Protocol Filename: | NMR_sample_preparation.docx |
| Processing Storage Conditions: | 4℃ |
| Extract Storage: | 4℃ |
Analysis:
| MB Sample ID: | SA202854 |
| Analysis ID: | AN003460 |
| Analysis Type: | NMR |
| Num Factors: | 7 |
| Num Metabolites: | 62 |
| Units: | area under peak |
NMR:
| NMR ID: | NM000234 |
| Analysis ID: | AN003460 |
| Instrument Name: | 850 MHz Bruker Avance III |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D-1H |
| Spectrometer Frequency: | 850 MHz |
| NMR Probe: | 5 mm CPTCI 1H-13C/15N/D Z-GRD Z117769/0013 |
| NMR Solvent: | H2O+D2O |
| NMR Tube Size: | 5-mm |
| Pulse Sequence: | CPMGPR1D |
| Pulse Width: | 10.95 |
| Receiver Gain: | 128 |
| Temperature: | 25 |
| Number Of Scans: | 128 |
| Acquisition Time: | 1.9268 s |
| Relaxation Delay: | 4.0 s |
| Spectral Width: | 17006.8 Hz |
| Num Data Points Acquired: | 32 K data points |
| Chemical Shift Ref Std: | lactate 1.33 |
| Binned Increment: | 0.002 |
| Binned Data Excluded Range: | δ5.88–5.65 and δ5.12–4.68 |
| NMR Results File: | Binned_data_ppm.csv |
