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MB Sample ID: SA203054
| Local Sample ID: | m14a |
| Subject ID: | SU002202 |
| Subject Type: | Bacteria |
| Subject Species: | Bacteroides xylanisolvens |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002202 |
| Subject Type: | Bacteria |
| Subject Species: | Bacteroides xylanisolvens |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| m14a | SA203054 | FL025000 | 1 | Cage |
| m14a | SA203054 | FL025000 | Btheta | Microbiota |
| m14a | SA203054 | FL025000 | NAD-precursor deficient | Diet |
| m14a | SA203054 | FL025000 | NA | Age (months) |
| m14a | SA203054 | FL025000 | NA | WLZ |
| m14a | SA203054 | FL025000 | NA | Health phenotype |
Collection:
| Collection ID: | CO002195 |
| Collection Summary: | Cecal contents were collected on 10 uL plastic inoculation loops at the time of sacrifice, snap frozen in liquid nitrogen, and stored at -80 °C. Aliquots were kept frozen, removed from the loops and weighed. |
| Sample Type: | Cecum |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR002214 |
| Treatment Summary: | Germ-free C57BL/6J mice were reared in plastic flexible film gnotobiotic isolators (Class Biologically Clean) at 23 °C under a strict 12-hour light cycle (lights on at 0600h, off at 1800h). Animals were weaned onto an autoclaved, low-fat, plant polysaccharide-rich chow (catalog number 2018S, Envigo) that was administered ad libitum. Five days prior to colonization, 8-week-old male mice were switched to the NAD precursor-sufficient diet; 2 days later, they were either placed on a NAD precursor-deficient diet, or a NA-supplemented diet or were continued on the NAD precursor-sufficient diet. |
Sample Preparation:
| Sampleprep ID: | SP002208 |
| Sampleprep Summary: | 10 uL of ice-cold methanol was added per mg of sample, which was then thawed on ice. The mixture was subsequently shaken for two minutes in a BioSpec bead beater. Samples were then centrifuged for 10 minutes at 3,000 x g at 4 °C; a 150 uL aliquot of the supernatant was transferred to a new tube containing 150 uL ice-cold chloroform, then mixed by pulse vortexing and centrifuged for 10 minutes at 3,000 x g at 4 °C. 100 uL of the methanol fraction was transferred to a new tube containing 100 uL of ice-cold chloroform, mixed by pulse-vortexing, and centrifuged for 10 minutes at 3,000 x g at 4 °C. 70 uL was transferred to a new tube and lyophilized in a Labconco SpeedVac. |
Combined analysis:
| Analysis ID | AN003465 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1290 Infinity |
| Column | Waters Atlantis C18 |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Agilent 6490 QQQ |
| Ion Mode | POSITIVE |
| Units | pmol/mg |
Chromatography:
| Chromatography ID: | CH002559 |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Waters Atlantis C18 |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS003226 |
| Analysis ID: | AN003465 |
| Instrument Name: | Agilent 6490 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Metabolites were quantified by MassHunter quantitative analysis tool (Agilent) |
| Ion Mode: | POSITIVE |
