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MB Sample ID: SA204660
Local Sample ID: | H9 |
Subject ID: | SU002217 |
Subject Type: | Mammal |
Subject Species: | Papio hamadryas |
Taxonomy ID: | 9557 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002217 |
Subject Type: | Mammal |
Subject Species: | Papio hamadryas |
Taxonomy ID: | 9557 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
H9 | SA204660 | FL025116 | Nistplasma | type |
Collection:
Collection ID: | CO002210 |
Collection Summary: | The NIST plasma metabolomics dataset consisted of 150 replicate samples which were bought from commercial vendors. The 12 batched datasets were pooled, aligned, and processed using open source software MS-DIAL (v4.6). The second dataset was generated from metabolic profiling of 45 baboon plasma samples collected from 35 females in the age range of 6-23 years and 10 males in the same age range. All 45 plasma samples were analyzed using an untargeted EI-GC-MS approach as described above. The third dataset consists of another EI-GC-MS analysis of metabolites extracted from 47 liver biopsy samples collected from the same adult healthy baboons as the plasma which included 39 females and 8 males in the age range of 6-23 years. |
Sample Type: | Liver |
Treatment:
Treatment ID: | TR002229 |
Treatment Summary: | For the baboon study, normal life course baboons were fed control chow diet |
Sample Preparation:
Sampleprep ID: | SP002223 |
Sampleprep Summary: | 15 μL of plasma or liver samples were subjected to sequential solvent extraction, once each with 1 mL of acetonitrile: isopropanol: water (3:3:2) and 500 μL of acetonitrile: water (1:1) mixtures at 4°C [14]. An internal standard, adonitol (2 μL from 10 mg/ml stock) was added to each aliquot prior to the extraction. The extracts were dried under vacuum at 4°C prior to chemical derivatization (silylation reactions). Blank tubes without samples, were treated similarly as sample tubes and added to account for background noise and other sources of contamination. Samples and blanks were sequentially derivatized with meth-oxyamine hydrochloride (MeOX) and 1% TMCS in N-methyl-N-trimethylsilyl-trifluoroacetamide (MSTFA) or 1% TMCS containing N-(t-butyldimethylsilyl)-N-methyltrifluoroacetamide (MTBSTFA) as described elsewhere [15]. Briefly, the steps involved addition of 20 μL of MeOX (20 mg mL-1) in pyridine incu-bated at 55°C for 60 min followed by trimethylsilylation at 60°C for 60 min after adding 80 μL MTBSTFA. |
Combined analysis:
Analysis ID | AN003487 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Thermo Trace 1310 |
Column | Thermo Scientific Trace GOLD TG-5SIL-MS |
MS Type | EI |
MS instrument type | QTRAP |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE |
Units | Normalized Peak abundances |
Chromatography:
Chromatography ID: | CH002574 |
Instrument Name: | Thermo Trace 1310 |
Column Name: | Thermo Scientific Trace GOLD TG-5SIL-MS |
Chromatography Type: | GC |
MS:
MS ID: | MS003248 |
Analysis ID: | AN003487 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | QTRAP |
MS Type: | EI |
MS Comments: | Data acquisition and instrument control were carried out using Xcalibur 4.3 and Trace-Finder 4.1 softwares MS-DIAL |
Ion Mode: | POSITIVE |