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MB Sample ID: SA206365
Local Sample ID: | WT-1 |
Subject ID: | SU002239 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | AppNL-G-F or wild type mice |
Age Or Age Range: | 7 months old |
Gender: | Male and female |
Animal Animal Supplier: | RIKEN Center for Brain Science |
Animal Light Cycle: | 12 hours light and dark cycle |
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Subject:
Subject ID: | SU002239 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | AppNL-G-F or wild type mice |
Age Or Age Range: | 7 months old |
Gender: | Male and female |
Animal Animal Supplier: | RIKEN Center for Brain Science |
Animal Light Cycle: | 12 hours light and dark cycle |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
WT-1 | SA206365 | FL025286 | Wild type | Model |
WT-1 | SA206365 | FL025286 | B. breve MCC1274 | Intervention |
Collection:
Collection ID: | CO002232 |
Collection Summary: | Plasma samples were obtained from AD-like model and wild-type mice orally administered B. breve MCC1274 or saline for 4 months. |
Sample Type: | Blood (plasma) |
Storage Conditions: | -80℃ |
Treatment:
Treatment ID: | TR002251 |
Treatment Summary: | Three-month-old AppNL-G-F and wild type mice were randomly assigned to a vehicle group and a probiotic group. The vehicle group received saline, and the probiotic group received B. breve MCC1274 (1 × 10^9 cfu/200 μl saline/mouse) orally 5 times a week for 4 months. |
Treatment Compound: | Bifidobacterium breve MCC1274 |
Treatment Route: | oral administration |
Treatment Dosevolume: | 1 × 10^9 cfu/200 μl saline |
Treatment Vehicle: | saline |
Sample Preparation:
Sampleprep ID: | SP002245 |
Sampleprep Summary: | For CE-FTMS, 40 µL of plasma was added to 160 µL of methanol containing internal standards at 0°C, 120 µL of Milli-Q water was added and mixed thoroughly, and 240 µL of this mixture was added to a Millipore 5 kDa cutoff filter (ULTRAFREE MC PLHCC, HMT) at 9100 × g for 120 min at 4°C to remove macromolecules. The filtrate was evaporated to dryness under vacuum, dissolved in 40 µL Milli-Q water, and donated to HMT's ω Scan package using capillary electrophoresis Fourier transform mass spectrometry (CE-FTMS).For LC-TOFMS, 80 µL of plasma was added to 240 µL of 1% formic acid/acetonitrile containing internal standards at 0ºC. The mixture was centrifuged at 2,300 x g, 4ºC for 5 min, then centrifuged at 2,300 x g, 4ºC for 5 min, followed by centrifugation of a hybrid SPE phospholipid cartridge (30 mg/mL of hybrid SPE-phospholipid, 30 mg/mL of (SUPELCO) and filtered to remove phospholipids. The filtrate was then evaporated to dryness under nitrogen, dissolved in 80 μL of 50% isopropanol (v/v), and metabolomic analysis was performed using liquid chromatography time-of-flight mass spectrometry (LC-TOFMS). |
Combined analysis:
Analysis ID | AN003526 | AN003527 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Normal phase | Reversed phase |
Chromatography system | Agilent 1260 | Agilent 1200 |
Column | fused silica capillary (50 μm i.d. × 80 cm total length; Polymicro Technologies, Inc., Phoenix, AZ) | ODS |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Triple TOF |
MS instrument name | Thermo Q Exactive Plus Orbitrap | Agilent 6210 TOF |
Ion Mode | UNSPECIFIED | UNSPECIFIED |
Units | peak area | peak area |
Chromatography:
Chromatography ID: | CH002604 |
Instrument Name: | Agilent 1260 |
Column Name: | fused silica capillary (50 μm i.d. × 80 cm total length; Polymicro Technologies, Inc., Phoenix, AZ) |
Internal Standard: | H3304-1002, Human Metabolome Technologies, Inc. (HMT), Tsuruoka, Yamagata, Japan |
Solvent A: | commercial electrophoresis buffer (H3301-1001; I3302-1023 for cation; anion analyses, respectively, HMT) |
Chromatography Type: | Normal phase |
Chromatography ID: | CH002605 |
Instrument Name: | Agilent 1200 |
Column Name: | ODS |
Internal Standard: | H3304-1002, Human Metabolome Technologies, Inc. (HMT), Tsuruoka, Yamagata, Japan |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS003284 |
Analysis ID: | AN003526 |
Instrument Name: | Thermo Q Exactive Plus Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | The spectrometer was scanned from m/z 60 to 900 in positive mode, and from m/z 70 to 1,050 in negative mode |
Ion Mode: | UNSPECIFIED |
MS ID: | MS003285 |
Analysis ID: | AN003527 |
Instrument Name: | Agilent 6210 TOF |
Instrument Type: | Triple TOF |
MS Type: | ESI |
MS Comments: | The spectrometer was scanned from m/z 50 to 1,000 and peaks were extracted using MasterHands, automatic integration software (Keio University, Tsuruoka, Yamagata, Japan) |
Ion Mode: | UNSPECIFIED |