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MB Sample ID: SA208869
| Local Sample ID: | CC_567_FF2 |
| Subject ID: | SU002256 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002256 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Male and female |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| CC_567_FF2 | SA208869 | FL025470 | Cecum | Organ |
| CC_567_FF2 | SA208869 | FL025470 | SPF | Treatment |
| CC_567_FF2 | SA208869 | FL025470 | Fiber-free | Diet |
Collection:
| Collection ID: | CO002249 |
| Collection Summary: | Cecal metabolome of specific-pathogen-free mice fed with five distinct rodent diets with varying fiber content and source. |
| Sample Type: | Cecum |
Treatment:
| Treatment ID: | TR002268 |
| Treatment Summary: | Experiments were performed with 6-week old female BALB/c mice (Charles River Laboratories, France), housed in the specific-pathogen-free (SPF) facility of the Luxembourg Institute of Health. Mice were fed on their respective diets for 40 days and then sacrificed by cervical dislocation. |
Sample Preparation:
| Sampleprep ID: | SP002262 |
| Sampleprep Summary: | Cecal contents were lyophilized using a VD-800R lyophilizer (TAITEC) for 24 hours. Freeze-dried samples were disrupted with 3.0-mm Zirconia Beads (Biomedical Science) by vigorous shaking (1,500 rpm for 10 min) using Shake Master (Biomedical Science). Cecal metabolites were extracted using the methanol:chloroform:water extraction protocol. |
Combined analysis:
| Analysis ID | AN003555 | AN003556 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | CE | CE |
| Chromatography system | Agilent CE | Agilent CE |
| Column | Fused-silica,50um(cation) | COSMO(+),50um(anion |
| MS Type | ESI | ESI |
| MS instrument type | Other | Other |
| MS instrument name | Agilent CE-TOFMS | Agilent CE-TOFMS |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | nmol/g | nmol/g |
Chromatography:
| Chromatography ID: | CH002626 |
| Instrument Name: | Agilent CE |
| Column Name: | Fused-silica,50um(cation) |
| Chromatography Type: | CE |
| Chromatography ID: | CH002627 |
| Instrument Name: | Agilent CE |
| Column Name: | COSMO(+),50um(anion |
| Chromatography Type: | CE |
MS:
| MS ID: | MS003312 |
| Analysis ID: | AN003555 |
| Instrument Name: | Agilent CE-TOFMS |
| Instrument Type: | Other |
| MS Type: | ESI |
| MS Comments: | In-house software (MasterHands) was used for peak area integration and annotation. |
| Ion Mode: | POSITIVE |
| MS ID: | MS003313 |
| Analysis ID: | AN003556 |
| Instrument Name: | Agilent CE-TOFMS |
| Instrument Type: | Other |
| MS Type: | ESI |
| MS Comments: | In-house software (MasterHands) was used for peak area integration and annotation. |
| Ion Mode: | NEGATIVE |
