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MB Sample ID: SA208908
| Local Sample ID: | AF20_BC_197 |
| Subject ID: | SU002258 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002258 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Male and female |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| AF20_BC_197 | SA208908 | FL025480 | Cecum | Organ |
| AF20_BC_197 | SA208908 | FL025480 | Germfree | Treatment |
| AF20_BC_197 | SA208908 | FL025480 | Fiber-free | Diet |
Collection:
| Collection ID: | CO002251 |
| Collection Summary: | Cecal metabolome of gnotobiotic (containing a 14-member synthetic human gut microbiota), and germ-free mice fed with two distinct rodent diets with varying fiber content. |
| Sample Type: | Cecum |
Treatment:
| Treatment ID: | TR002270 |
| Treatment Summary: | Germ-free BALB/c mice were bred in isolators and transferred to iso-cages prior to experimental feeding and/or 14SM colonization. Mice were fed on their respective diets for 40 days and then sacrificed by cervical dislocation. |
Sample Preparation:
| Sampleprep ID: | SP002264 |
| Sampleprep Summary: | Cecal contents were lyophilized using a VD-800R lyophilizer (TAITEC) for 24 hours. Freeze-dried samples were disrupted with 3.0-mm Zirconia Beads (Biomedical Science) by vigorous shaking (1,500 rpm for 10 min) using Shake Master (Biomedical Science). Cecal metabolites were extracted using the methanol:chloroform:water extraction protocol. |
Combined analysis:
| Analysis ID | AN003559 | AN003560 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | CE | CE |
| Chromatography system | Agilent CE | Agilent CE |
| Column | Fused-silica,50um(cation) | COSMO(+),50um(anion |
| MS Type | ESI | ESI |
| MS instrument type | Other | Other |
| MS instrument name | Agilent CE-TOFMS | Agilent CE-TOFMS |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | nmol/g | nmol/g |
Chromatography:
| Chromatography ID: | CH002630 |
| Instrument Name: | Agilent CE |
| Column Name: | Fused-silica,50um(cation) |
| Chromatography Type: | CE |
| Chromatography ID: | CH002631 |
| Instrument Name: | Agilent CE |
| Column Name: | COSMO(+),50um(anion |
| Chromatography Type: | CE |
MS:
| MS ID: | MS003316 |
| Analysis ID: | AN003559 |
| Instrument Name: | Agilent CE-TOFMS |
| Instrument Type: | Other |
| MS Type: | ESI |
| MS Comments: | In-house software (MasterHands) was used for peak area integration and annotation. |
| Ion Mode: | POSITIVE |
| MS ID: | MS003317 |
| Analysis ID: | AN003560 |
| Instrument Name: | Agilent CE-TOFMS |
| Instrument Type: | Other |
| MS Type: | ESI |
| MS Comments: | In-house software (MasterHands) was used for peak area integration and annotation. |
| Ion Mode: | NEGATIVE |
