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MB Sample ID: SA208963

Local Sample ID:19 NT 13C 2
Subject ID:SU002260
Subject Type:Other abiotic sample

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Subject:

Subject ID:SU002260
Subject Type:Other abiotic sample

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
19 NT 13C 2SA208963FL025490VEHGroup

Collection:

Collection ID:CO002253
Collection Summary:Treatment and sample collection summary Dulbecco’s Modified Eagle’s Medium (DMEM, Corning, NY) supplemented with 10% fetal bovine serum (Atlanta biologicals) were treated with or without 0.2 mM hydrogen peroxide for 30 min in room temperature in triplicate. After incubation samples were immediately frozen in -80C.
Sample Type:Media

Treatment:

Treatment ID:TR002272
Treatment Summary:Dulbecco’s Modified Eagle’s Medium (DMEM, Corning, NY) supplemented with 10% fetal bovine serum (Atlanta biologicals) were treated with or without 0.2 mM hydrogen peroxide for 30 min in room temperature in triplicate. After incubation samples were immediately frozen in -80C.

Sample Preparation:

Sampleprep ID:SP002266
Sampleprep Summary:Samples were extracted with methanol and in house cocktail of amino acids, acting as an internal standards. Samples were then homogenized and spun down, and supernatant was dried down. Dried down samples were reconstituted and transferred to LCMS vials, then injected on LCMS at 2ul per sample
Sampleprep Protocol ID:0.01.12.12
Processing Storage Conditions:4℃
Extraction Method:E.01
Extract Storage:-80℃
Sample Resuspension:50uL
Sample Spiking:500nM

Combined analysis:

Analysis ID AN003562
Analysis type MS
Chromatography type HILIC
Chromatography system Ulitmate 3000
Column SeQuant ZIC-pHILIC (150 x 4.6mm,5um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Thermo Q Exactive Orbitrap
Ion Mode UNSPECIFIED
Units N/L

Chromatography:

Chromatography ID:CH002633
Chromatography Summary:SeQuant ZIC-pHILIC 5um polymer, 150x2.1mm - Part No. 15046000001 Buffer A: 10mM ammonium carbonate in water, LCMS Grade Buffer B: Acetonitrile, LCMS Grade Rear Wash: 10% methanol in water, LCMS Grade Wash Buffer: water, LCMS Grade Injection Volume: 2uL
Methods Filename:L12
Instrument Name:Ulitmate 3000
Column Name:SeQuant ZIC-pHILIC (150 x 4.6mm,5um)
Column Pressure:1800
Column Temperature:25
Flow Rate:0.100ml/min
Injection Temperature:4
Internal Standard:ISTD (500nM amino acid cocktail)
Solvent A:100% water; 10 mM ammonium carbonate
Solvent B:100% acetonitrile
Chromatography Type:HILIC

MS:

MS ID:MS003319
Analysis ID:AN003562
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:M.13 Polar metabolites (pHILIC Hybrid).
Ion Mode:UNSPECIFIED
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