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MB Sample ID: SA210176
| Local Sample ID: | F1423 |
| Subject ID: | SU002277 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Age Or Age Range: | E18.5 |
| Gender: | Male and female |
| Animal Housing: | Vivarium University of Colorado Anschutz Medical Campus |
| Animal Light Cycle: | 12/12 |
| Animal Feed: | CH; D12450B; Research Diets, New Brunswick, NJ, USA or HFD; D12451; Research Diets |
| Animal Water: | Water or treated with 1.25 mg/L PQQ |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002277 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Age Or Age Range: | E18.5 |
| Gender: | Male and female |
| Animal Housing: | Vivarium University of Colorado Anschutz Medical Campus |
| Animal Light Cycle: | 12/12 |
| Animal Feed: | CH; D12450B; Research Diets, New Brunswick, NJ, USA or HFD; D12451; Research Diets |
| Animal Water: | Water or treated with 1.25 mg/L PQQ |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| F1423 | SA210176 | FL025659 | HFD | Diet |
Collection:
| Collection ID: | CO002270 |
| Collection Summary: | Livers were excised and snap frozen then sent to the Metabolomics Core at the University of Colorado Anschutz Medical Campus for analysis following standard protocols. |
| Sample Type: | Liver |
| Storage Conditions: | -80℃ |
| Collection Vials: | Cryotubes |
| Storage Vials: | Cryotubes |
Treatment:
| Treatment ID: | TR002289 |
| Treatment Summary: | Dams were fed either chow (CH) or high fat diet (HFD), with or without PQQ in drinking water. |
| Treatment: | HFD and PQQ |
| Treatment Compound: | BioPQQ |
| Treatment Route: | Drinking water, ad libitem |
| Treatment Dose: | 1.25 mg/L |
| Treatment Doseduration: | 6 weeks |
| Treatment Vehicle: | drinking water |
Sample Preparation:
| Sampleprep ID: | SP002283 |
| Sampleprep Summary: | Tissue was homogenized and lipids extracted following standard protocols at the Metabolomics Core at the University of Colorado Anschutz Medical Campus. |
| Sampleprep Protocol Filename: | Fetal_and_weanling_lipid_protocols.pdf |
Combined analysis:
| Analysis ID | AN003586 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Vanquish |
| Column | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | UNSPECIFIED |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH002650 |
| Chromatography Summary: | The analytical platform employed a Vanquish UHPLC system (Thermo Fisher Scientific, San Jose, CA, USA) coupled online to a Q Exactive mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA). Lipidomics analyses were performed as described in previous method papers and application notes. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7640979/, https://www.haematologica.org/article/view/9990, https://pubmed.ncbi.nlm.nih.gov/31119660/ |
| Methods Filename: | Fetal_and_weanling_lipid_protocols.pdf |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS003341 |
| Analysis ID: | AN003586 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | See attached file |
| Ion Mode: | UNSPECIFIED |
| Analysis Protocol File: | Fetal_and_weanling_lipid_protocols.pdf |
