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MB Sample ID: SA211828
| Local Sample ID: | 09C5 |
| Subject ID: | SU002298 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002298 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male and female |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 09C5 | SA211828 | FL025831 | C(Term3) | Diet_phase |
| 09C5 | SA211828 | FL025831 | C5 | Timepoint |
Collection:
| Collection ID: | CO002291 |
| Collection Summary: | Human fecal metabolome profiles under 3 different dietary terms. During the first phase (term 1), there was no restriction on diet, but consumption of medicinal drugs and dietary supplements were prohibited. In the second phase (term 2), subjects ate provided meals. The menus of meals were different every time. For the last phase (term 3), subjects continuously ate an identical meal 15 times, three times a day over 5 days. Commercially available box meals, frozen foods, fruits, sandwiches, rice balls, retort foods were provided in term 2 and 3. Throughout the trial, subjects collected their feces and froze them as soon as they could. After which, the frozen fecal samples were shipped frozen to the Institute for Advanced Biosciences, Keio University, Yamagata, Japan, for metabolome and metagenome analysis. |
| Sample Type: | Feces |
Treatment:
| Treatment ID: | TR002310 |
| Treatment Summary: | Human fecal metabolome profiles under 3 different dietary terms. During the first phase (term 1), there was no restriction on diet, but consumption of medicinal drugs and dietary supplements were prohibited. In the second phase (term 2), subjects ate provided meals. The menus of meals were different every time. For the last phase (term 3), subjects continuously ate an identical meal 15 times, three times a day over 5 days. Commercially available box meals, frozen foods, fruits, sandwiches, rice balls, retort foods were provided in term 2 and 3. Throughout the trial, subjects collected their feces and froze them as soon as they could. After which, the frozen fecal samples were shipped frozen to the Institute for Advanced Biosciences, Keio University, Yamagata, Japan, for metabolome and metagenome analysis. |
Sample Preparation:
| Sampleprep ID: | SP002304 |
| Sampleprep Summary: | Fecal samples were freeze-dried, and then metabolites were extracted using the methanol:chloroform:water extraction protocol. |
Combined analysis:
| Analysis ID | AN003616 | AN003617 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | CE | CE |
| Chromatography system | Agilent CE | Agilent CE |
| Column | Fused-silica,50um(cation) | COSMO(+),50um(anion |
| MS Type | ESI | ESI |
| MS instrument type | Other | Other |
| MS instrument name | Agilent CE-TOFMS | Agilent CE-TOFMS |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | μmol/g | μmol/g |
Chromatography:
| Chromatography ID: | CH002671 |
| Instrument Name: | Agilent CE |
| Column Name: | Fused-silica,50um(cation) |
| Internal Standard: | Methionine sulfone |
| Chromatography Type: | CE |
| Chromatography ID: | CH002672 |
| Instrument Name: | Agilent CE |
| Column Name: | COSMO(+),50um(anion |
| Internal Standard: | D-camphor-10-sulfonic acid |
| Chromatography Type: | CE |
MS:
| MS ID: | MS003367 |
| Analysis ID: | AN003616 |
| Instrument Name: | Agilent CE-TOFMS |
| Instrument Type: | Other |
| MS Type: | ESI |
| MS Comments: | In-house software (MasterHands) was used for peak area integration and annotation. |
| Ion Mode: | POSITIVE |
| MS ID: | MS003368 |
| Analysis ID: | AN003617 |
| Instrument Name: | Agilent CE-TOFMS |
| Instrument Type: | Other |
| MS Type: | ESI |
| MS Comments: | In-house software (MasterHands) was used for peak area integration and annotation. |
| Ion Mode: | NEGATIVE |
