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MB Sample ID: SA211889
Local Sample ID: | D2_Glucose_1 |
Subject ID: | SU002301 |
Subject Type: | Other organism |
Subject Species: | Aurantiochytrium limacinum |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002301 |
Subject Type: | Other organism |
Subject Species: | Aurantiochytrium limacinum |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
D2_Glucose_1 | SA211889 | FL025841 | Glucose | Treatment |
Collection:
Collection ID: | CO002294 |
Collection Summary: | Marine thraustochytrid Aurantiochytrium limacinum was a native isolate obtained as a generous gift from ICT-Mumbai, India. It was cultivated in YPD medium with the optimized concentration of 3% (w/v) glucose and glycerol at 20 C for a duration of 96 h. |
Sample Type: | Cultured cells |
Treatment:
Treatment ID: | TR002313 |
Treatment Summary: | It was cultivated in YPD medium with the optimized concentration of 3% (w/v) glucose and glycerol at 20 C for a duration of 96 h. |
Treatment: | Glucose and Glycerol |
Sample Preparation:
Sampleprep ID: | SP002307 |
Sampleprep Summary: | Quenched cells were resuspended in 1 mL of ice-cold methanol/ethanol/chloroform(2:6:2), followed by sonication of resuspended cells in sonication bath for 15 min. Later, these samples were centrifuged at 10,000×g for 15 min at 4 °C to get rid of cell debris. The supernatant was filtered using a 0.2-µm filter. One hundred microlitres of supernatant was taken and dried under nitrogen stream. The dried leftover was dissolved in 10 µL of freshly prepared methoxyamine hydrochloride solution (40 mg mL−1 in pyridine) and incubated at 30 °C for 90 min with shaking. To the above solution, 90 µL of N-methyl-N-(trimethylsilyl)trifluoroacetamide was added and incubated at 37 °C for 30 min. The samples were centrifuged at 14,000×g for 3 min, and the supernatant was taken for the GC-MS/MS analysis. |
Processing Storage Conditions: | Described in summary |
Sample Derivatization: | MSTFA |
Sample Spiking: | Nonadecanoic acid |
Combined analysis:
Analysis ID | AN003624 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Agilent 7890A |
Column | Agilent DB5-MS (30m x 0.25mm, 0.25um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Agilent 7890A |
Ion Mode | POSITIVE |
Units | Area |
Chromatography:
Chromatography ID: | CH002679 |
Instrument Name: | Agilent 7890A |
Column Name: | Agilent DB5-MS (30m x 0.25mm, 0.25um) |
Chromatography Type: | GC |
MS:
MS ID: | MS003375 |
Analysis ID: | AN003624 |
Instrument Name: | Agilent 7890A |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | Mass Hunter |
Ion Mode: | POSITIVE |