Return to study ST002215 main page
MB Sample ID: SA211896
| Local Sample ID: | D2_Glycerol_1 |
| Subject ID: | SU002301 |
| Subject Type: | Other organism |
| Subject Species: | Aurantiochytrium limacinum |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002301 |
| Subject Type: | Other organism |
| Subject Species: | Aurantiochytrium limacinum |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| D2_Glycerol_1 | SA211896 | FL025842 | Glycerol | Treatment |
Collection:
| Collection ID: | CO002294 |
| Collection Summary: | Marine thraustochytrid Aurantiochytrium limacinum was a native isolate obtained as a generous gift from ICT-Mumbai, India. It was cultivated in YPD medium with the optimized concentration of 3% (w/v) glucose and glycerol at 20 C for a duration of 96 h. |
| Sample Type: | Cultured cells |
Treatment:
| Treatment ID: | TR002313 |
| Treatment Summary: | It was cultivated in YPD medium with the optimized concentration of 3% (w/v) glucose and glycerol at 20 C for a duration of 96 h. |
| Treatment: | Glucose and Glycerol |
Sample Preparation:
| Sampleprep ID: | SP002307 |
| Sampleprep Summary: | Quenched cells were resuspended in 1 mL of ice-cold methanol/ethanol/chloroform(2:6:2), followed by sonication of resuspended cells in sonication bath for 15 min. Later, these samples were centrifuged at 10,000×g for 15 min at 4 °C to get rid of cell debris. The supernatant was filtered using a 0.2-µm filter. One hundred microlitres of supernatant was taken and dried under nitrogen stream. The dried leftover was dissolved in 10 µL of freshly prepared methoxyamine hydrochloride solution (40 mg mL−1 in pyridine) and incubated at 30 °C for 90 min with shaking. To the above solution, 90 µL of N-methyl-N-(trimethylsilyl)trifluoroacetamide was added and incubated at 37 °C for 30 min. The samples were centrifuged at 14,000×g for 3 min, and the supernatant was taken for the GC-MS/MS analysis. |
| Processing Storage Conditions: | Described in summary |
| Sample Derivatization: | MSTFA |
| Sample Spiking: | Nonadecanoic acid |
Combined analysis:
| Analysis ID | AN003624 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 7890A |
| Column | Agilent DB5-MS (30m x 0.25mm, 0.25um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Agilent 7890A |
| Ion Mode | POSITIVE |
| Units | Area |
Chromatography:
| Chromatography ID: | CH002679 |
| Instrument Name: | Agilent 7890A |
| Column Name: | Agilent DB5-MS (30m x 0.25mm, 0.25um) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS003375 |
| Analysis ID: | AN003624 |
| Instrument Name: | Agilent 7890A |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Mass Hunter |
| Ion Mode: | POSITIVE |
