Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA217028

Local Sample ID:	LQC11
Subject ID:	SU002340
Subject Type:	Mammal
Subject Species:	Papio hamadryas
Taxonomy ID:	9557

Select appropriate tab below to view additional metadata details:

Subject:

Subject ID:	SU002340
Subject Type:	Mammal
Subject Species:	Papio hamadryas
Taxonomy ID:	9557

Factors:

Local Sample ID	MB Sample ID	Factor Level ID	Level Value	Factor Name
LQC11	SA217028	FL026327	NIST PLASMA	Sample type

Collection:

Collection ID:	CO002333
Collection Summary:	The liver, skeletal muscle and plasma samples consisted of 32 samples each which were obtained from post-pubertal offspring (3-6.5y at sample collection) of MO mothers (n=19) and from control animals born to mothers fed a standard diet (CON, n=13). The datasets were generated from metabolic profiling of these tissues and plasma samples collected from 17 females in the age range of 3-6.5 years and 13 males in the same age range. All were analyzed using an untargeted EI-GC-MS approach as described above.
Sample Type:	Liver

Treatment:

Treatment ID:	TR002352
Treatment Summary:	We analyzed liver and muscle biopsies and plasma samples obtained from the post-pubertal offspring (3-6.5 years old at sample collection) of MO mothers (n=19, 10 females and 9 males) and from control animals born to mothers fed a standard diet (n=13, 6 females and 7 males). All offspring animals ate normal chow diet after weaning so differences between groups are due to the impact of the maternal intrauterine environment.

Treatment ID:

TR002352

Treatment Summary:

We analyzed liver and muscle biopsies and plasma samples obtained from the post-pubertal offspring (3-6.5 years old at sample collection) of MO mothers (n=19, 10 females and 9 males) and from control animals born to mothers fed a standard diet (n=13, 6 females and 7 males). All offspring animals ate normal chow diet after weaning so differences between groups are due to the impact of the maternal intrauterine environment.

Sample Preparation:

Sampleprep ID:	SP002346
Sampleprep Summary:	15 μL of plasma, liver or skeletal muscle samples were subjected to sequential solvent extraction, once each with 1 mL of acetonitrile: isopropanol: water (3:3:2) and 500 μL of acetonitrile: water (1:1) mixtures at 4°C [14]. An internal standard, adonitol (2 μL from 10 mg/ml stock) was added to each aliquot prior to the extraction. The extracts were dried under vacuum at 4°C prior to chemical derivatization (silylation reactions). Blank tubes without samples, were treated similarly as sample tubes and added to account for background noise and other sources of contamination. Samples and blanks were sequentially derivatized with meth-oxyamine hydrochloride (MeOX) and 1% TMCS in N-methyl-N-trimethylsilyl-trifluoroacetamide (MSTFA) or 1% TMCS containing N-(t-butyldimethylsilyl)-N-methyltrifluoroacetamide (MTBSTFA) as described elsewhere [15]. Briefly, the steps involved addition of 20 μL of MeOX (20 mg mL-1) in pyridine incu-bated at 55°C for 60 min followed by trimethylsilylation at 60°C for 60 min after adding 80 μL MTBSTFA.

Sampleprep ID:

SP002346

Sampleprep Summary:

15 μL of plasma, liver or skeletal muscle samples were subjected to sequential solvent extraction, once each with 1 mL of acetonitrile: isopropanol: water (3:3:2) and 500 μL of acetonitrile: water (1:1) mixtures at 4°C [14]. An internal standard, adonitol (2 μL from 10 mg/ml stock) was added to each aliquot prior to the extraction. The extracts were dried under vacuum at 4°C prior to chemical derivatization (silylation reactions). Blank tubes without samples, were treated similarly as sample tubes and added to account for background noise and other sources of contamination. Samples and blanks were sequentially derivatized with meth-oxyamine hydrochloride (MeOX) and 1% TMCS in N-methyl-N-trimethylsilyl-trifluoroacetamide (MSTFA) or 1% TMCS containing N-(t-butyldimethylsilyl)-N-methyltrifluoroacetamide (MTBSTFA) as described elsewhere [15]. Briefly, the steps involved addition of 20 μL of MeOX (20 mg mL-1) in pyridine incu-bated at 55°C for 60 min followed by trimethylsilylation at 60°C for 60 min after adding 80 μL MTBSTFA.

Combined analysis:

Analysis ID	AN003682
Analysis type	MS
Chromatography type	GC
Chromatography system	Thermo Trace 1310
Column	Thermo Scientific Trace GOLD TG-5SIL-MS
MS Type	EI
MS instrument type	QTRAP
MS instrument name	Thermo Q Exactive Orbitrap
Ion Mode	POSITIVE
Units	Normalized Peak Intensities

Chromatography:

Chromatography ID:	CH002730
Instrument Name:	Thermo Trace 1310
Column Name:	Thermo Scientific Trace GOLD TG-5SIL-MS
Chromatography Type:	GC

MS:

MS ID:	MS003433
Analysis ID:	AN003682
Instrument Name:	Thermo Q Exactive Orbitrap
Instrument Type:	QTRAP
MS Type:	EI
MS Comments:	Data acquisition and instrument control were carried out using Xcalibur 4.3 and Trace-Finder 4.1 softwares MS-DIAL
Ion Mode:	POSITIVE