Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA217102

Local Sample ID:	ASPC-GATP-5
Subject ID:	SU002341
Subject Type:	Cultured cells
Subject Species:	Homo sapiens
Taxonomy ID:	9606

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Subject:

Subject ID:	SU002341
Subject Type:	Cultured cells
Subject Species:	Homo sapiens
Taxonomy ID:	9606

Factors:

Local Sample ID	MB Sample ID	Factor Level ID	Level Value	Factor Name
ASPC-GATP-5	SA217102	FL026331	50µM gemcitabine and 40µM ATP for 72h	Treatment

Collection:

Collection ID:	CO002334
Collection Summary:	After 72 hours of drug treatment, the cells were washed twice with ice PBS and scraped in 80% ice methanol
Sample Type:	Tumor cells

Treatment:

Treatment ID:	TR002353
Treatment Summary:	Cells were evenly divided into 3 groups of 6 discs each. 24h after cell inoculation, gemcitabine administration group(GATP) was treated with gemcitabine with final concentration of 50μM for 72h and ATP with a final concentration of 40μM, Meanwhile, the control group (CATP) was given the same volume of DMSO with 40μM ATP. One plate of cells was taken from each group for counting, and the rest were collected for metabolite extraction

Treatment ID:

TR002353

Treatment Summary:

Cells were evenly divided into 3 groups of 6 discs each. 24h after cell inoculation, gemcitabine administration group(GATP) was treated with gemcitabine with final concentration of 50μM for 72h and ATP with a final concentration of 40μM, Meanwhile, the control group (CATP) was given the same volume of DMSO with 40μM ATP. One plate of cells was taken from each group for counting, and the rest were collected for metabolite extraction

Sample Preparation:

Sampleprep ID:	SP002347
Sampleprep Summary:	The cells were cultured as described above and fixed with 1 ml of 80% ice-cold menthol after being washed twice with ice-cold PBS. The cells were scraped from the plates, and 0.5-mm beads were added to process the cells by grinding and shaking. The supernatants were collected after centrifugation and deproteinized by mixing with 800μL acetonitrile on ice. Then, the supernatants collected and spun down under nitrogen at room temperature. The samples were resuspended in 100 μL of distilled H2O, and 5 μL was used for LC-TQ-MS-based metabolome assay.

Sampleprep ID:

SP002347

Sampleprep Summary:

The cells were cultured as described above and fixed with 1 ml of 80% ice-cold menthol after being washed twice with ice-cold PBS. The cells were scraped from the plates, and 0.5-mm beads were added to process the cells by grinding and shaking. The supernatants were collected after centrifugation and deproteinized by mixing with 800μL acetonitrile on ice. Then, the supernatants collected and spun down under nitrogen at room temperature. The samples were resuspended in 100 μL of distilled H2O, and 5 μL was used for LC-TQ-MS-based metabolome assay.

Combined analysis:

Analysis ID	AN003683	AN003684
Analysis type	MS	MS
Chromatography type	Reversed phase	Reversed phase
Chromatography system	Agilent 1290 Infinity	Agilent 1290 Infinity
Column	Waters Acquity HSS T3 (100 x 2.1mm,1.8um)	Waters Acquity HSS T3 (100 x 2.1mm,1.8um)
MS Type	ESI	ESI
MS instrument type	Triple quadrupole	Triple quadrupole
MS instrument name	Agilent 6495 QQQ	Agilent 6495 QQQ
Ion Mode	POSITIVE	NEGATIVE
Units	counts	counts

Chromatography:

Chromatography ID:	CH002731
Instrument Name:	Agilent 1290 Infinity
Column Name:	Waters Acquity HSS T3 (100 x 2.1mm,1.8um)
Chromatography Type:	Reversed phase

MS:

MS ID:	MS003434
Analysis ID:	AN003683
Instrument Name:	Agilent 6495 QQQ
Instrument Type:	Triple quadrupole
MS Type:	ESI
MS Comments:	Agilent MassHunter Workstation Data Acquisition Agilent MassHunter
Ion Mode:	POSITIVE

MS ID:	MS003435
Analysis ID:	AN003684
Instrument Name:	Agilent 6495 QQQ
Instrument Type:	Triple quadrupole
MS Type:	ESI
MS Comments:	Agilent MassHunter Workstation Data Acquisition Agilent MassHunter
Ion Mode:	NEGATIVE