Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA217384

Local Sample ID:	M18
Subject ID:	SU002348
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Gender:	Male and female

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Subject:

Subject ID:	SU002348
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Gender:	Male and female

Factors:

Local Sample ID	MB Sample ID	Factor Level ID	Level Value	Factor Name
M18	SA217384	FL026370	M	Gender
M18	SA217384	FL026370	Obe	Group

Collection:

Collection ID:	CO002341
Collection Summary:	This longitudinal cohort study is an analysis of fecal samples collected from obese children before and after a 2-month weight reduction program that consisted of three visits to the hospital.
Sample Type:	Feces

Treatment:

Treatment ID:	TR002360
Treatment Summary:	Participants completed the questionnaires on general lifestyle (the time spent studying and using electronic devices, the duration and frequency of regular exercise, the presence of easily accessible locations to exercise, and their mode of transportation to school) and eating habits (meal duration, the consumption of late-night snacks, the consumption of breakfast, and the intake of sugar-sweetened beverages) and submitted them at the first hospital visit.

Treatment ID:

TR002360

Treatment Summary:

Participants completed the questionnaires on general lifestyle (the time spent studying and using electronic devices, the duration and frequency of regular exercise, the presence of easily accessible locations to exercise, and their mode of transportation to school) and eating habits (meal duration, the consumption of late-night snacks, the consumption of breakfast, and the intake of sugar-sweetened beverages) and submitted them at the first hospital visit.

Sample Preparation:

Sampleprep ID:	SP002354
Sampleprep Summary:	Frozen serum samples were thawed on ice. For preparation of the serum, a 50 µL sample was extracted using 1 mL of N2-degassed 1st extraction solution. Then, the samples were mixed for 10 min and centrifuged for 10 min at 18945 RCF and 4°C. The supernatant was dried for 6 hours at 45°C. The dried samples were re-extracted with 2nd extraction solution. Then, the extracted samples were redried for 8 hours under the same conditions used in the first extraction step. The dried samples were derivatized with methoxyamine at 30°C for 90 min and subsequently trimethylsilylated with a mixture of fatty acid methyl ester, which is used for the retention time index, in N-methyl-N-(trimethylsilyl)-trifluoroacetamide at 70°C for 45 min.

Sampleprep ID:

SP002354

Sampleprep Summary:

Frozen serum samples were thawed on ice. For preparation of the serum, a 50 µL sample was extracted using 1 mL of N2-degassed 1st extraction solution. Then, the samples were mixed for 10 min and centrifuged for 10 min at 18945 RCF and 4°C. The supernatant was dried for 6 hours at 45°C. The dried samples were re-extracted with 2nd extraction solution. Then, the extracted samples were redried for 8 hours under the same conditions used in the first extraction step. The dried samples were derivatized with methoxyamine at 30°C for 90 min and subsequently trimethylsilylated with a mixture of fatty acid methyl ester, which is used for the retention time index, in N-methyl-N-(trimethylsilyl)-trifluoroacetamide at 70°C for 45 min.

Combined analysis:

Analysis ID	AN003695
Analysis type	MS
Chromatography type	GC
Chromatography system	Agilent 7890B
Column	Restek Rtx-5Sil (30m x 0.25mm,0.25um)
MS Type	EI
MS instrument type	GC-TOF
MS instrument name	Leco Pegasus HT TOF
Ion Mode	NEGATIVE
Units	intensity

Chromatography:

Chromatography ID:	CH002738
Instrument Name:	Agilent 7890B
Column Name:	Restek Rtx-5Sil (30m x 0.25mm,0.25um)
Chromatography Type:	GC

MS:

MS ID:	MS003446
Analysis ID:	AN003695
Instrument Name:	Leco Pegasus HT TOF
Instrument Type:	GC-TOF
MS Type:	EI
MS Comments:	The LECO Corporation ChromaTOF®-GC software (v4.72) was used for data acquisition and extraction. This included computation of baseline (offset of 1; above the noise) and automatic entering the number of data points for smoothing. The software's Statistical Compare was used to align peaks. The MS spectral matching was performed using the NIST and Wiley9 libraries. Also, retention index method was used for identification.
Ion Mode:	NEGATIVE