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MB Sample ID: SA217499
| Local Sample ID: | SH6 3 |
| Subject ID: | SU002351 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002351 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| SH6 3 | SA217499 | FL026404 | Healthy 6 | Sample Type |
Collection:
| Collection ID: | CO002344 |
| Collection Summary: | Participants were asked to chew on a piece of sterile parafilm and expectorate stimulated saliva into a sterile universal tube until approximately 6mls was collected. |
| Sample Type: | Saliva |
| Storage Conditions: | -20℃ |
Treatment:
| Treatment ID: | TR002363 |
| Treatment Summary: | No treatment |
Sample Preparation:
| Sampleprep ID: | SP002357 |
| Sampleprep Summary: | nuclear magnetic resonance (NMR) analysis on thawed saliva aliquots (500 l, n=3 per participant) was performed using a previously published method 11. Briefly, samples were centrifuged for 5 minutes at 13,000 RPM to remove cell debris. The supernatant was removed for NMR analysis and mixed at a ratio of 4:1 with sodium trimethylsilyl propionate (TSP) buffer and added to a 5mm NMR tube (Bruker, Coventry, UK). Spectra were acquired on a 600 MHz NMR spectrophotometer (Bruker). The spectra were processed on TopSpin (Bruker) to correct the phase and baseline, and to calibrate the TSP peak to 0 ppm. The identity of metabolites were assigned and the concentration (mM) obtained from spectra using Chenomx NMR Suite version 8.5 (Chenomix Ltd, Canada). |
| Processing Storage Conditions: | On ice |
Analysis:
| MB Sample ID: | SA217499 |
| Analysis ID: | AN003700 |
| Laboratory Name: | NMR Centre, KCL |
| Analysis Type: | NMR |
| Num Factors: | 12 |
| Num Metabolites: | 16 |
| Units: | mM |
NMR:
| NMR ID: | NM000246 |
| Analysis ID: | AN003700 |
| Instrument Name: | Bruker AVANCE III HD 600 MHz NMR spectrometer |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D-1H |
| Spectrometer Frequency: | 600MHz |
| NMR Probe: | TCI (1H/19F)/13C/15N Prodigy Cryoprobe |
| NMR Solvent: | TSP |
| NMR Tube Size: | 5mm |
| Pulse Sequence: | PROJECT |
| Temperature: | 298K |
| Number Of Scans: | 128 |
| Acquisition Time: | 2.62 s |
| Relaxation Delay: | 4 s |
| Spectral Width: | 20.8 ppm |
| Real Data Points: | 65536 points |
