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MB Sample ID: SA226658

Local Sample ID:FIT2-LKO_03
Subject ID:SU002389
Subject Type:Mammal
Subject Species:Mus musculus

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Subject:

Subject ID:SU002389
Subject Type:Mammal
Subject Species:Mus musculus

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
FIT2-LKO_03SA226658FL027496Liver KnockoutGenotype

Collection:

Collection ID:CO002382
Collection Summary:All mice fasted 2 hours prior to sacrifice. Mice were euthanized with isoflurane and tissues were collected.
Sample Type:Liver

Treatment:

Treatment ID:TR002401
Treatment Summary:No treatment

Sample Preparation:

Sampleprep ID:SP002395
Sampleprep Summary:Liver (~100mg) was homogenized in 1 mL of PBS using a Bead Mill Homogenizer (VWR). Lipids were extracted, according to the Folch method.
Processing Storage Conditions:On ice
Extract Storage:-80℃

Combined analysis:

Analysis ID AN003763 AN003764
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000
Column Thermo Accucore C30 (250 x 2.1mm,3um) Thermo Accucore C30 (250 x 2.1mm,3um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units Area Area

Chromatography:

Chromatography ID:CH002783
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Thermo Accucore C30 (250 x 2.1mm,3um)
Chromatography Type:Reversed phase

MS:

MS ID:MS003506
Analysis ID:AN003763
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Mass spectrometry data analysis was performed using LipidSearch version 4.1 SP (Thermo Fisher Scientific). The results were exported to R-Studio where quality control was performed using pairwise correlations between replicates, a principal component analysis comparing sample groups, as well as retention time plot analysis to verify elution clustering within lipid classes. All identified lipids were included for subsequent analyses if they fulfilled the following LipidSearch-based criteria: 1) reject equal to zero, 2) main grade A OR main grade B AND APvalue<0.01 for at least three replicates, and 3) no missing values across all samples.
Ion Mode:POSITIVE
  
MS ID:MS003507
Analysis ID:AN003764
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Mass spectrometry data analysis was performed using LipidSearch version 4.1 SP (Thermo Fisher Scientific). The results were exported to R-Studio where quality control was performed using pairwise correlations between replicates, a principal component analysis comparing sample groups, as well as retention time plot analysis to verify elution clustering within lipid classes. All identified lipids were included for subsequent analyses if they fulfilled the following LipidSearch-based criteria: 1) reject equal to zero, 2) main grade A OR main grade B AND APvalue<0.01 for at least three replicates, and 3) no missing values across all samples.
Ion Mode:NEGATIVE
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