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MB Sample ID: SA227375
| Local Sample ID: | 48 |
| Subject ID: | SU002407 |
| Subject Type: | Other organism |
| Subject Species: | Thalassiosira pseudonana |
| Taxonomy ID: | 296543 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002407 |
| Subject Type: | Other organism |
| Subject Species: | Thalassiosira pseudonana |
| Taxonomy ID: | 296543 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 48 | SA227375 | FL027676 | 14.0 | Factor_1 (temperature) |
| 48 | SA227375 | FL027676 | 0.0 | Factor_2 (bacteria presence) |
Collection:
| Collection ID: | CO002400 |
| Collection Summary: | 320 mL of diatom cells were collected by filtering the culture onto 2.0-µm-pore-size PCTE membrane filters (MilliporeSigma Isopore). Filters were kept in 50 mL tubes (Falcon) and stored at -80oC until processing. |
| Collection Protocol Filename: | 2_Collection protocol__UGA_phytoplankton_Oct2022.docx |
| Sample Type: | Algae |
Treatment:
| Treatment ID: | TR002419 |
| Treatment Summary: | Six treatments of a marine diatom strain Thalassiosira pseudonana CCMP1335 were prepared: treatments incubated axenically at either 14, 20, or 28 oC, and treatments co-cultured with a bacterial strain Ruegeria pomeroyi DSS-3 at the corresponding temperatures (four replicates for each). L1 media was used with NaH13CO3 as a source of bicarbonate. The diatom used for the co-cultured treatments was B12 stressed to emphasize the known co-existing system. The light cycle consisted of 16 h light (120 µmol photons m-2 s-1) and 8 h of dark. |
| Treatment Protocol Filename: | 3_Treatment protocol__UGA_phytoplankton_Oct2022.docx |
Sample Preparation:
| Sampleprep ID: | SP002413 |
| Sampleprep Summary: | Phytoplankton cells were removed from filters using a sonicator SLPe (Branson) in ultra-pure water (Millipore), concentrated by a lyophilizer (Labconco), and kept -80oC until further processing. The samples were mixed with 600 µL of 30 mmol L-1 sodium phosphate buffer (18 mmol L-1 NaHPO4, 12 mmol L-1, pH 7.4) and an internal standard of 2,2-dimethyl-2-silapentane-5-sulfonate-d6 (DSS, 1 mmol L-1), vortexed at 4oC for 5 minutes, centrifuged at 20,800 rcf using an ultracentrifuge 5417C (Eppendorf) at 4oC for 10 minutes, and supernatants were transferred to 5-mm NMR tubes (NORELL). |
| Sampleprep Protocol Filename: | 4_Sample preparation protocol__UGA_phytoplankton_Oct2022.docx |
Analysis:
| MB Sample ID: | SA227375 |
| Analysis ID: | AN003788 |
| Analysis Type: | NMR |
| Results File: | ST002321_AN003788_Results.txt |
| Units: | Intensity |
NMR:
| NMR ID: | NM000254 |
| Analysis ID: | AN003788 |
| Instrument Name: | Bruker NEO |
| Instrument Type: | CW-NMR |
| NMR Experiment Type: | Other |
| Spectrometer Frequency: | 600 MHz |
