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MB Sample ID: SA232163
| Local Sample ID: | S20 |
| Subject ID: | SU002423 |
| Subject Type: | Invertebrate |
| Subject Species: | Drosophila melanogaster |
| Taxonomy ID: | 7227 |
| Age Or Age Range: | 15 day old flies |
| Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU002423 |
| Subject Type: | Invertebrate |
| Subject Species: | Drosophila melanogaster |
| Taxonomy ID: | 7227 |
| Age Or Age Range: | 15 day old flies |
| Gender: | Male and female |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| S20 | SA232163 | FL029129 | UAS-synj-AuxWT/F956x | Genotype |
Collection:
| Collection ID: | CO002416 |
| Collection Summary: | Heads of 15DO flies were homogenized in 100 µl D-PBS (Dulbecco’s phosphate-buffered saline without Mg2+ and Ca2+) and stored at -80°C until processed for MS analysis by Lipotype. Fifteen fly heads were pooled from three independent crosses for each analysis, and mass spectrometry was performed on n=7 for wild-type, n≥4 analyses for dAuxWT/WT, dAuxRG/RG, dAuxWT/F956x and dAuxRG/F956x and n=3 for dAuxWT/F956x and dAuxRG/F956x overexpressing Synj. |
| Sample Type: | Insect tissue |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR002435 |
| Treatment Summary: | In this study we did not apply any additional treatment(s) to the samples. |
Sample Preparation:
| Sampleprep ID: | SP002429 |
| Sampleprep Summary: | Lipids were extracted using chloroform and methanol (1). Prior to lipid extraction, samples were spiked with lipid class-specific internal standards. After drying and resuspending in an appropriate MS acquisition mixture, lipid extracts were infused directly in QExactive mass spectrometer (Thermo Fisher Scientific) with TriVersa NanoMate ion source (Advion Biosciences). Samples are analyzed in both positive and negative ion modes, with MS resolution Rm/z=200=280000 and MSMS resolution Rm/z=200=17500, in a single acquisition. Sample preparations and analysis were done by Lipotype. (1) Sampaio, J. L. et al. Membrane lipidome of an epithelial cell line. Proceedings of the National Academy of Sciences 108, 1903–1907 (2011). |
Combined analysis:
| Analysis ID | AN003814 |
|---|---|
| Analysis type | MS |
| Chromatography type | None (Direct infusion) |
| Chromatography system | none |
| Column | none |
| MS Type | ESI |
| MS instrument type | QTRAP |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE |
| Units | pMol |
Chromatography:
| Chromatography ID: | CH002821 |
| Instrument Name: | none |
| Column Name: | none |
| Chromatography Type: | None (Direct infusion) |
MS:
| MS ID: | MS003556 |
| Analysis ID: | AN003814 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | Samples are analyzed in both positive and negative ion modes, with MS resolution Rm/z=200=280000 and MSMS resolution Rm/z=200=17500, in a single acquisition. Acquired data was processed using lipid identification software based on LipidXplorer (1). Data post-processing and normalization were performed by Lipotype using a developed data management system. Data analysis was performed using GraphPad Prism software 9.3.1. Pmol values (obtained by MS) of individual lipid species were transformed into a fraction of the total PA, DAG, PI, PC, PE, and PS lipids in the sample. (1)Herzog, R. et al. Lipidxplorer: A software for consensual cross-platform lipidomics. PLoS ONE 7, e29851 (2012). |
| Ion Mode: | POSITIVE |
