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MB Sample ID: SA235670
Local Sample ID: | JM SQ 2 |
Subject ID: | SU002437 |
Subject Type: | Bacteria |
Subject Species: | Escherichia coli |
Taxonomy ID: | 562 |
Genotype Strain: | BW25113 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002437 |
Subject Type: | Bacteria |
Subject Species: | Escherichia coli |
Taxonomy ID: | 562 |
Genotype Strain: | BW25113 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
JM SQ 2 | SA235670 | FL029240 | SQ | Factor |
Collection:
Collection ID: | CO002430 |
Collection Summary: | Cells were metabolically arrested by infusing with ice-cold PBS and washed with PBS. |
Sample Type: | Bacterial cells |
Treatment:
Treatment ID: | TR002449 |
Treatment Summary: | E. coli adapted to growth on the relevant substrate was grown on M9 minimal medium containing either Glc or SQ as a sole carbon source until mid-log phase was achieved. |
Sample Preparation:
Sampleprep ID: | SP002443 |
Sampleprep Summary: | Cell pellets were resuspended in chilled extraction solution (500 µL) comprised of 3:1 MeOH: H2O and (13C5, 15N)valine (1 mM, 0.5 µL) and (13C6)sorbitol (1 mM, 0.5 µL) as internal standards. The suspensions were subjected to freeze-thaw cycles to facilitate lysis of the cells (30 s in liquid N2, 30 s in a dry ice/EtOH bath for 10 cycles), and shaken (9000 rpm, 10 min, 2°C). The samples were then centrifuged (12700 rpm, 5 min, 1°C). The cell lysate supernatant was transferred into glass inserts and dried. All samples were washed with MeOH (50 µL). All samples were methoximated with methoxylamine hydrochloride solution (30 mg/mL in pyridine, 20 µL) for 2 h at 37°C, followed by trimethylsilyation in BSTFA + 1% TMCS (20 µL) for 1 h at 37°C with continuous mixing. Samples were incubated at rt for 1 h prior to GC-MS analysis. |
Combined analysis:
Analysis ID | AN003833 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Shimadzu GC-2010 |
Column | J&W DB-5 (30 m × 0.25mm,1.00um) |
MS Type | EI |
MS instrument type | Triple quadrupole |
MS instrument name | Shimazu TQ8040 |
Ion Mode | UNSPECIFIED |
Units | a.u. |
Chromatography:
Chromatography ID: | CH002838 |
Instrument Name: | Shimadzu GC-2010 |
Column Name: | J&W DB-5 (30 m × 0.25mm,1.00um) |
Chromatography Type: | GC |
MS:
MS ID: | MS003575 |
Analysis ID: | AN003833 |
Instrument Name: | Shimazu TQ8040 |
Instrument Type: | Triple quadrupole |
MS Type: | EI |
MS Comments: | Please see attached file. |
Ion Mode: | UNSPECIFIED |