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MB Sample ID: SA237393
Local Sample ID: | WT3 |
Subject ID: | SU002466 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6N |
Age Or Age Range: | 6-weeks old |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU002466 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6N |
Age Or Age Range: | 6-weeks old |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
WT3 | SA237393 | FL029405 | Wild-type | Genotype |
Collection:
Collection ID: | CO002459 |
Collection Summary: | Mice were deeply anesthetized and perfused intracardially with saline containing 1 mM MOPS (pH 7.4). Liver was rapidly removed, frozen on liquid nitrogen and stored at –80°C until further processing. |
Sample Type: | Liver |
Storage Conditions: | -80℃ |
Treatment:
Treatment ID: | TR002478 |
Treatment Summary: | No treatment in this study. |
Sample Preparation:
Sampleprep ID: | SP002472 |
Sampleprep Summary: | Tissues with internal standards were homogenized with ice-cold methanol and extracted by the Bligh–Dyer method using chloroform and methanol to separate and recover the water-soluble fractions. The supernatant was filtered through a 5-kDa cut-off filter (Ultrafree-MC-PLHCC, Human Metabolome Technologies) at 9,100 × g for 2 h at 4 °C, dried in a centrifugal evaporator at 1,500 rpm at 1,000 Pa, and resuspended in 50 μl of ultrapure water before metabolome analysis. |
Combined analysis:
Analysis ID | AN003873 | AN003874 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | CE | CE |
Chromatography system | Agilent 7100 CE | Agilent 7100 CE |
Column | Fused silica capillary i.d. 50 μm × 80 cm | Fused silica capillary i.d. 50 μm × 80 cm |
MS Type | ESI | ESI |
MS instrument type | Other | Other |
MS instrument name | Agilent 6224 TOF | Agilent 6490 QQQ |
Ion Mode | POSITIVE | NEGATIVE |
Units | nmol | nmol |
Chromatography:
Chromatography ID: | CH002870 |
Instrument Name: | Agilent 7100 CE |
Column Name: | Fused silica capillary i.d. 50 μm × 80 cm |
Chromatography Type: | CE |
MS:
MS ID: | MS003614 |
Analysis ID: | AN003873 |
Instrument Name: | Agilent 6224 TOF |
Instrument Type: | Other |
MS Type: | ESI |
MS Comments: | The spectrometer was scanned from m/z 50 to 1,000. Peaks were extracted using automatic integration software MasterHands (ver.2.16.0.15 Keio University). Putative metabolites were assigned based on the m/z value and migration time using Human Metabolomic Technologies’ standard library and known–unknown peak library. Peak areas were normalized to internal standards and sample amounts. |
Ion Mode: | POSITIVE |
MS ID: | MS003615 |
Analysis ID: | AN003874 |
Instrument Name: | Agilent 6490 QQQ |
Instrument Type: | Other |
MS Type: | ESI |
MS Comments: | The spectrometer was scanned from m/z 50 to 1,000. Peaks were extracted using automatic integration software (MassHunter Quantitative Analysis B.06.00, Agilent Technologies). Putative metabolites were assigned based on the m/z value and migration time using Human Metabolomic Technologies’ standard library and known–unknown peak library. Peak areas were normalized to internal standards and sample amounts. |
Ion Mode: | NEGATIVE |